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Human Plexin A1 Antibody

Catalog #: MAB6536
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB6536
MAB6536-SP
Detection of Human Plexin A1 by Western Blot.
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Product Details
Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Plexin A1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Plexin A1 in direct ELISAs. In Western blots, no cross-reactivity with recombinant human Plexin A4, B1, B2, B3, C1, D1, recombinant mouse Plexin A1, A2, or A3 is observed.
Source
Monoclonal Mouse IgG1 Clone # 659807
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Plexin A1
Asn986-Leu1152
Accession # Q9UIW2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Plexin A1 antibody by Western Blot. View Larger

Detection of Human Plexin A1 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human Plexin A1 Monoclonal Antibody (Catalog # MAB6536) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Plexin A1 at approximately 210 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Plexin A1 antibody in Human Dendritic Cells by Immunocytochemistry (ICC). View Larger

Plexin A1 in Human Dendritic Cells. Plexin A1 was detected in immersion fixed immature human dendritic cells using Mouse Anti-Human Plexin A1 Monoclonal Antibody (Catalog # MAB6536) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Plexin A1

Plexin A1 is an approximately 200 kDa type I transmembrane member of the Plexin family of Semaphorin signal transducers. It consists of a 1218 amino acid (aa) extracellular domain with one Sema domain, a spacer, and four tandem IPT/TIG domains, a 21 aa transmembrane segment, and a 631 aa cytoplasmic domain. Plexin A1 binds the transmembrane Semaphorin 6D directly and binds the Class 3 secreted Semaphorins indirectly via Neuropilin-1 and -2. It mediates cytoskeletal remodeling that results in cell migration and axon repulsion. Within aa 964-1152 (in the IPT/TIG region), human Plexin A1 shares 93% aa sequence identity with mouse and rat Plexin A1.

Entrez Gene IDs
5361 (Human); 18844 (Mouse)
Alternate Names
NOV; NOVP; Plexin A1; PLEXIN-A1; PLXN1; PLXNA1

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Citation for Human Plexin A1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A Potential Role of Semaphorin 3A during Orthodontic Tooth Movement
    Authors: S ?en, CJ Lux, R Erber
    International Journal of Molecular Sciences, 2021-08-02;22(15):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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