Human/Rat PGD2 Synthase/PTGDS Antibody

Catalog #: MAB10099 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10099-100
MAB10099-SP

Detection of Human and Rat PGD2 Synthase/PTGDS by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Rat PGD2 Synthase/PTGDS Antibody Summary

Species Reactivity

Human, Rat

Specificity

Detects human and rat PGD2 Synthase/PTGDS in direct ELISA and Western blots.

Source

Monoclonal Mouse IgG_2A Clone # 997303

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human PGD2 Synthase/PTGDS
Met1-Gln190
Accession # P41222

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Immunohistochemistry

5-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Rat PGD2 Synthase/PTGDS by Western Blot. Western blot shows lysates of human heart tissue and rat brain tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Rat PGD2 Synthase/PTGDS Monoclonal Antibody (Catalog # MAB10099) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PGD2 Synthase/PTGDS at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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PGD2 Synthase/PTGDS in Human Brain. PGD2 Synthase/PTGDS was detected in immersion fixed paraffin-embedded sections of human brain (caudate nucleus) using Mouse Anti-Human/Rat PGD2 Synthase/PTGDS Monoclonal Antibody (Catalog # MAB10099) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PGD2 Synthase/PTGDS

Prostaglandin-H2 D-isomerase or PGD2 synthase (PTGDS) is an enzyme that catalyzes the conversion of Prostaglandin H2 (PGH2) to Prostaglandin D2 (PGD2), a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. Prostaglandin D2 synthase is used clinically as a diagnostic marker for Cerebrospinal fluid rhinorrhea, important in the assessment of head trauma severity. It has been also proposed that the urinary and serum levels pf PTGDS may be also a marker of renal damage in diabetes mellitus and hypertension.

Long Name

Prostaglandin D2 Synthase

Entrez Gene IDs

5730 (Human); 25526 (Rat)

Alternate Names

Beta-trace protein; Cerebrin-28; EC 5.3.99.2; Glutathione-independent PGD synthase; glutathione-independent PGD synthetase; lipocalin-type prostaglandin D synthase; Lipocalin-type prostaglandin-D synthase; L-PGDS; PDS; PGD2 Synthase; PGD2; PGDS2; PGDSLPGDS; PH2DISO; prostaglandin D synthase; prostaglandin D2 synthase (21kD, brain); prostaglandin D2 synthase 21kDa (brain); Prostaglandin-D2 synthase; prostaglandin-H2 D-isomerase; PTGDS