Human WASP Antibody

Catalog #: MAB30701 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB30701
MAB30701-SP

Detection of Human WASP by Western Blot.

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Human WASP Antibody Summary

Species Reactivity

Human

Specificity

Detects human WASP in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 336918

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human WASP
Lys230-Gln310 (predicted)
Accession # P42768

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human WASP by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2.5 µg/mL of Mouse Anti-Human WASP Monoclonal Antibody (Catalog # MAB30701) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for WASP at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: WASP

WASP (Wiskott-Aldrich Syndrome Protein) is a 54 kDa protein belonging to the WASP/SCAR/WAVE family of proteins involved in actin polymerization. It is a highly conserved protein and is expressed selectively in hematopoietic stem cell-derived lineages. WASP contains multiple domains with unique functions. Mutations in WASP are associated with diseases of the hematopoietic/ immunologic system.

Long Name

Wiskott-Aldrich Syndrome Protein

Entrez Gene IDs

7454 (Human); 22376 (Mouse)

Alternate Names

IMD2THC; Thrombocytopenia 1; WAS; WASP; WiskTHC1; wiskthrombocytopenia 1 (X-linked)