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Mouse CCR3 Antibody

Catalog #: MAB1551
9 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1551-100
MAB1551-SP
Product Details
Citations (9)
FAQs
Supplemental Products
Reviews (1)
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCR3 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCR3. Specifically stains mouse CCR3 transfectants but not the parental cell lines. Does not cross‑react with Y3 cells transfected with human CCR3.
Source
Monoclonal Rat IgG2A Clone # 83103
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Y3 rat myeloid cell line transfected with mouse CCR3
Met1-Phe359
Accession # P51678
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
Mouse CCR3 transfected Y3 cells
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR3

CCR3 is a G protein-linked seven transmembrane receptor that is activated by several different chemokines. It is expressed on subsets of granulocytes, monocytes, T cells and epithelial cells.

Entrez Gene IDs
1232 (Human); 12771 (Mouse); 117027 (Rat); 448802 (Canine)
Alternate Names
b-chemokine receptor; CC chemokine receptor 3; C-C chemokine receptor type 3; C-C CKR-3; CCR3; CCR-3; CD193 antigen; CD193; chemokine (C-C motif) receptor 3; CKR3CC-CKR-3CMKBR3CD193; eosinophil CC chemokine receptor 3; Eosinophil eotaxin receptor; MGC102841

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Citations for Mouse CCR3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Effects of a herbal formulation, KGC3P, and its individual component, nepetin, on coal fly dust-induced airway inflammation
    Authors: E Saba, YS Lee, WK Yang, YY Lee, M Kim, SM Woo, K Kim, YS Kwon, TH Kim, D Kwak, YC Park, HJ Shin, CK Han, JW Oh, YC Lee, HS Kang, MH Rhee, SH Kim
    Sci Rep, 2020-08-20;10(1):14036.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. CCL28 promotes locomotor recovery after spinal cord injury via recruiting regulatory T cells
    Authors: P Wang, X Qi, G Xu, J Liu, J Guo, X Li, X Ma, H Sun
    Aging (Albany NY), 2019-09-26;11(18):7402-7415.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  3. Breast milk immune complexes are potent inducers of oral tolerance in neonates and prevent asthma development.
    Authors: Mosconi E, Rekima A, Seitz-Polski B
    Mucosal Immunol, 2010-05-19;3(5):461-74.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. CCR1 expression and signal transduction by murine BMMC results in secretion of TNF-alpha, TGFbeta-1 and IL-6.
    Authors: Fifadara NH, Aye CC, Raghuwanshi SK, Richardson RM, Ono SJ
    Int. Immunol., 2009-07-10;21(8):991-1001.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. CCR3 is a target for age-related macular degeneration diagnosis and therapy.
    Authors: Takeda A, Baffi JZ, Kleinman ME, Cho WG, Nozaki M, Yamada K, Kaneko H, Albuquerque RJ, Dridi S, Saito K, Raisler BJ, Budd SJ, Geisen P, Munitz A, Ambati BK, Green MG, Ishibashi T, Wright JD, Humbles AA, Gerard CJ, Ogura Y, Pan Y, Smith JR, Grisanti S, Hartnett ME, Rothenberg ME, Ambati J
    Nature, 2009-06-14;460(7252):225-30.
    Species: Mouse
    Sample Types: In Vivo, Vitreous Humor
    Applications: Neutralization
  6. Ablation of type I hypersensitivity in experimental allergic conjunctivitis by eotaxin-1/CCR3 blockade.
    Authors: Miyazaki D, Nakamura T, Ohbayashi M, Kuo CH, Komatsu N, Yakura K, Tominaga T, Inoue Y, Higashi H, Murata M, Takeda S, Fukushima A, Liu FT, Rothenberg ME, Ono SJ
    Int. Immunol., 2009-01-15;21(2):187-201.
    Species: Mouse
    Sample Types: In Vivo, Whole Tissue
    Applications: IHC-Fr, Neutralization
  7. CD11b+ myeloid cells are the key mediators of Th2 cell homing into the airway in allergic inflammation.
    Authors: Medoff BD, Seung E, Hong S, Thomas SY, Sandall BP, Duffield JS, Kuperman DA, Erle DJ, Luster AD
    J. Immunol., 2009-01-01;182(1):623-35.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Siglec-F antibody administration to mice selectively reduces blood and tissue eosinophils.
    Authors: Zimmermann N, McBride ML, Yamada Y, Hudson SA, Jones C, Cromie KD, Crocker PR, Rothenberg ME, Bochner BS
    Allergy, 2008-09-01;63(9):1156-63.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Flow Cytometry, Neutralization
  9. Toll-like receptor-2-mediated C-C chemokine receptor 3 and eotaxin-driven eosinophil influx induced by Mycobacterium bovis BCG pleurisy.
    Authors: D'Avila H, Almeida PE, Roque NR, Castro-Faria-Neto HC, Bozza PT
    Infect. Immun., 2006-12-11;75(3):1507-11.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization

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Reviews for Mouse CCR3 Antibody

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Mouse CCR3 Antibody
By Anonymous on 08/18/2016
Application: Flow Sample Tested: Bronchoalveolar lavage fluid (mouse),Bronchoalveolar lavage fluid Species: Mouse

3 step staining;
1. unconjugated CCR3 Ab
2. Biotinylated anti-rat IgG
3. Streptavidin-APC

Flow Cytometry Mouse CCR3 Antibody MAB1551