Mouse IGFBP-3 Antibody

Catalog #: MAB775
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB775
MAB775-SP

All IGFBP-3 Antibodies

Product Details

Citations (4)

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IGFBP-3 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IGFBP-3 in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant human IGFBP‑1, rhIGFBP‑2, rhIGFBP‑3, rhIGFBP‑4, rhIGFBP‑5, rhIGFBP‑6, recombinant mouse (rm) IGFBP‑2, rmIGFBP‑5, or rmIGFBP‑6.

Source

Monoclonal Rat IgG_2B Clone # 138202

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IGFBP‑3
Pro22-Gln291 (Arg250Gln, Gln259Arg, and Arg271Pro)
Accession # P47878

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse IGFBP‑3 (Catalog # 775-B3)
under non-reducing conditions only

Mouse IGFBP-3 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Mouse IGFBP‑3 Biotinylated Antibody (Catalog # BAF775)

Standard: Recombinant Mouse IGFBP-3 Protein, CF (Catalog # 775-B3)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGFBP-3

The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBPs, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF. Mouse IGFBP-3 cDNA encodes a 291 amino acid (aa) residue precursor protein with a putative 27 aa residue signal peptide that is processed to generate the 264 aa residue mature protein with three potential N-linked glycosylation sites. Mouse and human IGFBP-3 share 81% amino acid homology. Mouse IGFBP-3 is expressed in multiple tissues.

References

Schuller, A.G.P. et al. (1994) Mol. Cell. Endoc. 104:57.

Long Name

Insulin-like Growth Factor Binding Protein 3

Entrez Gene IDs

3486 (Human); 16009 (Mouse)

Alternate Names

acid stable subunit of the 140 K IGF complex; binding protein 29; binding protein 53; growth hormone-dependent binding protein; IBP-3; IBP3BP-53; IGF-binding protein 3; IGFBP3; IGFBP-3; insulin-like growth factor binding protein 3; insulin-like growth factor-binding protein 3

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse IGFBP-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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IGFBP-3 is a metastasis suppression gene in prostate cancer.
Authors: Mehta HH, Gao Q, Galet C
Cancer Res., 2011-06-22;71(15):5154-63.
Species: Mouse
Sample Types: Serum
Applications: ELISA Development
Evidence of a role for insulin-like growth factor binding protein (IGFBP)-3 in metabolic regulation.
Authors: Yamada PM, Mehta HH, Hwang D
Endocrinology, 2010-10-06;151(12):5741-50.
Species: Mouse
Sample Types: Serum
Applications: ELISA Development
Dietary feeding of silibinin inhibits prostate tumor growth and progression in transgenic adenocarcinoma of the mouse prostate model.
Authors: Raina K, Blouin MJ, Singh RP, Majeed N, Deep G, Varghese L, Glode LM, Greenberg NM, Hwang D, Cohen P, Pollak MN, Agarwal R
Cancer Res., 2007-11-15;67(22):11083-91.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Development
The ternary IGF complex influences postnatal bone acquisition and the skeletal response to intermittent parathyroid hormone.
Authors: Yakar S, Bouxsein ML, Canalis E, Sun H, Glatt V, Gundberg C, Cohen P, Hwang D, Boisclair Y, LeRoith D, Rosen CJ
J. Endocrinol., 2006-05-01;189(2):289-99.
Species: Mouse
Sample Types: Serum
Applications: ELISA Development