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Mouse Sca-1/Ly6 Antibody

Catalog #: MAB1226
12 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1226
MAB1226-SP
Detection of Sca‑1/Ly6 in Mouse Splenocytes by Flow Cytometry.
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Product Details
Citations (12)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Sca-1/Ly6 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Sca‑1/Ly6 in direct ELISAs.
Source
Monoclonal Rat IgG2A Clone # 177228
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Sca‑1/Ly6 C-terminally truncated Ly-6E allele
Leu27-Gly119
Accession # CAA28351
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Sca‑1/Ly6 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of Sca‑1/Ly6 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes gated on hematopoietic lineage negative cells were stained with Rat Anti-Mouse CD117/c-kit PE-conjugated Monoclonal Antibody (Catalog # FAB1356P) and either (A) Rat Anti-Mouse Sca-1/Ly6 Monoclonal Antibody (Catalog # MAB1226) or (B) Rat IgG2AIsotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

Immunocytochemistry Sca‑1/Ly6 antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

Sca‑1/Ly6 in Mouse Splenocytes. Sca-1/Ly6 was detected in immersion fixed mouse splenocytes using 10 µg/mL Rat Anti-Mouse Sca-1/Ly6 Monoclonal Antibody (Catalog # MAB1226) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Sca-1/Ly6

Stem Cell Antigen-1 (Sca-1) is encoded by the strain-specific Ly-6 E/A allelic gene. Its expression on multipotent hematopoietic stem cells (HSC) has been used as a marker of HSC in mice of both Ly-6 haplotypes (2, 3). This antibody is frequently used in combination with lineage depletion antibodies to identify and isolate murine HSC. Sca-1-positive HSC can be found in the adult bone marrow, fetal liver and mobilized peripheral blood and spleen in the adult animal (2‑7). However, Sca-1 has also been discovered in several non-hematopoietic tissues (1) and can be used to enrich progenitor cell populations other than HSC (8). It is suggested that Sca-1 could be involved in regulating both B and T cell activation (9‑12).

References
  1. Van de Rijn, M. et al. (1989) Proc. Natl. Acad. Sci. USA 86:4634.
  2. Spangrude, G.I. et al. (1988) Science 241:58.
  3. Spangrude, G.I. et al. (1993) Blood 82:3327.
  4. Morrison, S.J. et al. (1995) Proc. Natl. Acad. Sci. USA 92:10302.
  5. Kawamoto, H. et al. (1997) Int. Immunol. 9:1011.
  6. Yamamoto, Y. et al. (1996, Blood 88:445.
  7. Morrison, S.J. et al. (1997) Proc. Natl. Acad. Sci. USA 94:1908.
  8. Welm, B.E. et al. (2002) Dev. Biol. 245:42.
  9. Codias, E.K. et al. (1990) J. Immunol. 144:2197.
  10. Malek, T.R. et al. (1986) J. Exp. Med. 164:709.
  11. Codias, E.K. et al. (1990) J. Immunol. 145:1407.
  12. Flood, P.M. et al. (1990) J. Exp. Med. 172:115.
Long Name
Stem Cell Antigen-1/Lymphocyte Antigen 6
Entrez Gene IDs
110454 (Mouse)
Alternate Names
Ly6; Ly-6; Ly-6A.2; Ly-6A/E; Ly-6E.1; lymphocyte antigen 6 complex, locus A; lymphocyte antigen 6A-2/6E-1; Sca1; Sca-1; Stem cell antigen 1; TAP; T-cell-activating protein

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Citations for Mouse Sca-1/Ly6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Wnt/ beta -catenin signaling accelerates mouse lung tumorigenesis by imposing an embryonic distal progenitor phenotype on lung epithelium
    Authors: Eugenia C. Pacheco-Pinedo, Amy C. Durham, Kathleen M. Stewart, Ashley M. Goss, Min Min Lu, Francesco J. DeMayo et al.
    Journal of Clinical Investigation
  2. Perivascular localized cells commit erythropoiesis in PDGF-B-expressing solid tumors
    Authors: Hosaka K, Wang C, Zhang S et al.
    Cancer communications (London, England)
  3. Defining cell type-specific immune responses in a mouse model of allergic contact dermatitis by single-cell transcriptomics
    Authors: Liu, Y;Yin, M;Mao, X;Wu, S;Wei, S;Heng, S;Yang, Y;Huang, J;Guo, Z;Li, C;Ji, C;Hu, L;Liu, W;Zhang, LJ;
    eLife
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  4. Dynamic interplay between IL-1 and WNT pathways in regulating dermal adipocyte lineage cells during skin development and wound regeneration
    Authors: Sun, L;Zhang, X;Wu, S;Liu, Y;Guerrero-Juarez, CF;Liu, W;Huang, J;Yao, Q;Yin, M;Li, J;Ramos, R;Liao, Y;Wu, R;Xia, T;Zhang, X;Yang, Y;Li, F;Heng, S;Zhang, W;Yang, M;Tzeng, CM;Ji, C;Plikus, MV;Gallo, RL;Zhang, LJ;
    Cell reports
  5. Perivascular localized cells commit erythropoiesis in PDGF-B-expressing solid tumors
    Authors: Hosaka K, Wang C, Zhang S et al.
    Cancer communications (London, England)
  6. Multicolor quantitative confocal imaging cytometry
    Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
    Nat. Methods, 2017-11-13;15(1):39-46.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Tissue-engineered vascular grafts form neovessels that arise from regeneration of the adjacent blood vessel.
    Authors: Hibino N, Villalona G, Pietris N
    FASEB J., 2011-05-12;25(8):2731-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. BCL6 is critical for the development of a diverse primary B cell repertoire.
    Authors: Duy C, Yu JJ, Nahar R, Swaminathan S, Kweon SM, Polo JM, Valls E, Klemm L, Shojaee S, Cerchietti L, Schuh W, Jack HM, Hurtz C, Ramezani-Rad P, Herzog S, Jumaa H, Koeffler HP, de Alboran IM, Melnick AM, Ye BH, Muschen M
    J. Exp. Med., 2010-05-24;207(6):1209-21.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Magnetic surface-enhanced Raman spectroscopic (M-SERS) dots for the identification of bronchioalveolar stem cells in normal and lung cancer mice.
    Authors: Noh MS, Jun BH, Kim S, Kang H, Woo MA, Minai-Tehrani A, Kim JE, Kim J, Park J, Lim HT, Park SC, Hyeon T, Kim YK, Jeong DH, Lee YS, Cho MH
    Biomaterials, 2009-05-02;30(23):3915-25.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Intranasal HGF administration ameliorates the physiologic and morphologic changes in lung emphysema.
    Authors: Hegab AE, Kubo H, Yamaya M, Asada M, He M, Fujino N, Mizuno S, Nakamura T
    Mol. Ther., 2008-06-17;16(8):1417-26.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  11. Single Cell and Open Chromatin Analysis Reveals Molecular Origin of Epidermal Cells of the Skin
    Authors: Xiying Fan, Dongmei Wang, Jeremy Evan Burgmaier, Yudong Teng, Rose-Anne Romano, Satrajit Sinha et al.
    Developmental Cell
  12. Tgfbeta signaling is critical for maintenance of the tendon cell fate
    Authors: Tan GK, Pryce BA, Stabio A et al.
    Elife

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