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Mouse Siglec-F Antibody

Catalog #: MAB17061
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB17061-SP
MAB17061-500
MAB17061-100
Cell Adhesion Mediated by Siglec‑F and Neutralization by Mouse Siglec‑F Antibody.
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Citations (8)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Siglec-F Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Siglec-F in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Siglec-2 or -3, or recombinant human Siglec-5, -7, -9, -10, or -11 is observed.
Source
Monoclonal Rat IgG2A Clone # 238047
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Siglec-F
Asp18-Thr437
Accession # Q920G3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Neutralization
Measured by its ability to neutralize Siglec‑F-mediated adhesion of human red blood cells. Kelm, S. et al. (1994) Current Biology 4:965. The Neutralization Dose (ND50) is typically 0.1-0.5 µg/mL in the presence of 5 µg/mL Recombinant Mouse Siglec‑F Fc Chimera.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Adhesion Mediated by Siglec‑F and Neutralization by Mouse Siglec‑F Antibody. View Larger

Cell Adhesion Mediated by Siglec‑F and Neutralization by Mouse Siglec‑F Antibody. Recombinant Mouse Siglec-F Fc Chimera (Catalog # 1706-SF), immobilized onto a microplate, supports the adhesion of human red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Mouse Siglec-F Fc Chimera (5 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Siglec-F Monoclonal Antibody (Catalog # MAB17061). The ND50 is typically 0.1-0.5 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Siglec-F

Siglecs (1) (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins (2) belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding (3), followed by varying numbers of Ig-like C2-type domains (1, 4). Eleven human Siglecs have been cloned and characterized (1, 4). They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglec 5 to 11 (4‑6). To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. Siglec 5 to 11 share a high degree of sequence similarity with CD33/Siglec-3 both in their extracellular and intracellular regions. They are collectively referred to as CD33-related Siglecs. One remarkable feature of the CD33-related Siglecs is their differential expression pattern within the hematopoietic system (4, 5). This fact, together with the presence of two conserved immunoreceptor tyrosine-based inhibition motifs (ITIMs) in their cytoplasmic tails, suggests that CD33-related Siglecs are involved in the regulation of cellular activation within the immune system.

Mouse Siglec-F cDNA encodes a 569 amino acid polypeptide with a hydrophobic signal peptide, an N-terminal Ig-like V-type domain, three Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail (7). The expression of Siglec-F is restricted to the cells of myelomonocytic lineage. Mouse Siglec-F is likely an ortholog of human Siglec-5. Unlike many human CD33-related Siglecs, which show similar binding to both alpha 2,3- and alpha 2,6-linked sialic acids, mouse Siglec-F preferentially recognize alpha 2,3-linked sialic acid.

References
  1. Crocker, P.R. et al. (1998) Glycobiology 8:v.
  2. Powell, L.D. et al. (1995) J. Biol. Chem. 270:14243.
  3. May, A.R. et al. (1998) Mol. Cell 1998. 1:719.
  4. Crocker, P.R. and A. Varki (2001) Trends Immunol. 22:337.
  5. Crocker, P.R. et al. (2001) Immunology 103:137.
  6. Angata, T. et al. (2002) J. Biol Chem. 277:24466.
  7. Angata, T. et al. (2001) J. Biol Chem. 276:45128.
Long Name
Sialic Acid Binding Ig-like Lectin F
Entrez Gene IDs
233186 (Mouse)
Alternate Names
SAF2; sialic acid binding Ig like lectin 8; SIGLEC-8; SIGLEC8L; SiglecF; Siglec-F

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Citations for Mouse Siglec-F Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. The impact of Charcot-Leyden Crystal protein on mesothelioma chemotherapy: targeting eosinophils for enhanced chemosensitivity
    Authors: Willems, M;Hamaidia, M;Fontaine, A;Grégoire, M;Halkin, L;Vilanova Mañá, L;Terres, R;Jamakhani, M;Deshayes, S;Brostaux, Y;Heinen, V;Louis, R;Duysinx, B;Jean, D;Wasielewski, E;Scherpereel, A;Blanquart, C;Willems, L;
    EBioMedicine
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  2. Neonatal LTbetaR signaling is required for the accumulation of eosinophils in the inflamed adult mesenteric lymph node
    Authors: C Li, LA Ward, A Nguyen, E Lam, D Dasoveanu, M Ahmed, K Haniuda, MB Buechler, HH He, B Ludewig, KM McNagny, JL Gommerman
    Mucosal Immunology, 2022-02-18;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  3. Il4ra-independent vaginal eosinophil accumulation following helminth infection exacerbates epithelial ulcerative pathology of HSV-2 infection
    Authors: Alisha Chetty, Matthew G. Darby, Pia M. Vornewald, Mara Martín-Alonso, Anna Filz, Manuel Ritter et al.
    Cell Host & Microbe
  4. Fibroblast-derived IL-33 facilitates breast cancer metastasis by modifying the immune microenvironment and driving type-2 immunity
    Authors: Ophir Shani, Tatiana Vorobyov, Lea Monteran, Dor Lavie, Noam Cohen, Yael Raz et al.
    Cancer Research
  5. A role of eosinophils in mediating the anti-tumour effect of cryo-thermal treatment
    Authors: S Jia, W Li, P Liu, LX Xu
    Sci Rep, 2019-09-13;9(1):13214.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  6. Role of Eosinophils and Tumor Necrosis Factor Alpha in Interleukin-25-Mediated Protection from Amebic Colitis
    Authors: Z Noor, K Watanabe, MM Abhyankar, SL Burgess, EL Buonomo, CA Cowardin, WA Petri
    MBio, 2017-02-28;8(1):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  7. IL-33-mediated protection against experimental cerebral malaria is linked to induction of type 2 innate lymphoid cells, M2 macrophages and regulatory T cells.
    Authors: Besnard A, Guabiraba R, Niedbala W, Palomo J, Reverchon F, Shaw T, Couper K, Ryffel B, Liew F
    PLoS Pathog, 2015-02-06;11(2):e1004607.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Bioassay
  8. Eosinophils promote generation and maintenance of immunoglobulin-A-expressing plasma cells and contribute to gut immune homeostasis.
    Authors: Chu V, Beller A, Rausch S, Strandmark J, Zanker M, Arbach O, Kruglov A, Berek C
    Immunity, 2014-04-17;40(4):582-93.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization

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