Human Ajuba Antibody

Catalog #: AF5594 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5594
AF5594-SP

Detection of Human Ajuba by Western Blot.

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Human Ajuba Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human Ajuba in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Ajuba
Met1-Arg121
Accession # Q96IF1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Ajuba by Western Blot. Western blot shows lysates of PC-3 human prostate cancer cell line, A549 human lung carcinoma cell line, and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Human Ajuba Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5594) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Ajuba at approximately 55 to 60 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ajuba

Ajuba (an Urdu word meaning "curiosity") is a 55‑60 kDa member of the Zyxin/Ajuba family of proteins. Ajuba is found in keratinocytes, astrocytes and neurons, and exhibits multiple functions. It shuttles between the nucleus and cytosol, and participates in alpha -catenin:F-actin binding, beta -catenin binding and phosphorylation, p62 aPKC scaffold protein binding and NF-kappa B activation, and Aurora-A binding and phosphorylation. Human Ajuba is 538 amino acids (aa) in length. It contains a Pro-rich preLIM region that binds actin and Grb2 (aa 1‑335), an NES (aa 280‑287), and three LIM domains that bind multiple proteins (aa 336‑397, 401‑461 and 462‑530).

Entrez Gene IDs

84962 (Human); 16475 (Mouse); 85265 (Rat)

Alternate Names

Ajuba; JUB; jub, ajuba homolog (Xenopus laevis); MGC15563; protein ajuba