Human ALCAM/CD166 Antibody

Catalog #: AF656
5 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF656
AF656-SP

All ALCAM/CD166 Antibodies

Product Details

Citations (5)

Supplemental Products

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ALCAM/CD166 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ALCAM/CD166 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse ALCAM/CD166 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ALCAM/CD166
Trp28-Ala526
Accession # AAB59499

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human ALCAM/CD166 Fc Chimera (Catalog # 656-AL)

Adhesion Blockade

The adhesion of HuT 78 human cutaneous T cell lymphoma cells (5 x 10⁴ cells/well) to immobilized Recombinant Human CD6 Fc Chimera (Catalog # 627-CD, 10 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 25 µg/mL of the antibody.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ALCAM/CD166

ALCAM, activated leukocyte cell adhesion molecule, is a type I membrane glycoprotein and a member of the immunoglobulin supergene family. It is also known as CD166, MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. ALCAM is expressed on thymic epithelial cells, activated B and T cells, and monocytes. ALCAM can bind itself homotypically and is also capable of binding CD6, NgCAM, and other, as of yet, unidentified brain proteins. The ALCAM/CD6 interaction may be involved in T cell development and T cell regulation. Additionally, ALCAM/CD6 and ALCAM/NgCAM interactions may play roles in the nervous system. ALCAM has also been observed to be upregulated on highly metastasizing melanoma cell lines and may play a role in tumor migration. ALCAM is a 583 amino acid (aa) protein consisting of a 27 aa signal peptide, a 500 aa extracellular domain, a 24 aa transmembrane domain and a 32 aa cytoplasmic domain. The extracellular domain of ALCAM contains 5 Ig-like domains.

References

Bowen, M.A. et al. (1995) J. Exp. Med. 181:2213.
Aruffo, A. et al. (1997) Immunol. Today 18:498.
Degen, W.G. et al. (1998) Am. J. Pathol. 152:805.

Long Name

Activated Leukocyte Cell Adhesion Molecule

Entrez Gene IDs

214 (Human); 11658 (Mouse); 101867493 (Cynomolgus Monkey)

Alternate Names

activated leucocyte cell adhesion molecule; activated leukocyte cell adhesion moleculeFLJ38514; ALCAM; CD166 antigen; CD166; MEMDMGC71733

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human ALCAM/CD166 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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alpha2,6 Sialylation mediated by ST6GAL1 promotes glioblastoma growth
Authors: S Gc, K Tuy, L Rickenback, R Jones, A Chakrabort, CR Miller, EA Beierle, VS Hanumanthu, AN Tran, JA Mobley, SL Bellis, AB Hjelmeland
JCI Insight, 2022-11-08;7(21):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Floor-plate-derived netrin-1 is dispensable for commissural axon guidance
Authors: C Dominici, JA Moreno-Bra, SR Puiggros, Q Rappeneau, N Rama, P Vieugue, A Bernet, P Mehlen, A Chédotal
Nature, 2017-04-26;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Expression of the immunoglobulin superfamily cell adhesion molecules in the developing spinal cord and dorsal root ganglion.
Authors: Gu, Zirong, Imai, Fumiyasu, Kim, In Jung, Fujita, Hiroko, Katayama, Kei ichi, Mori, Kensaku, Yoshihara, Yoshihir, Yoshida, Yutaka
PLoS ONE, 2015-03-31;10(3):e0121550.
Applications: ICC
ALCAM regulates motility, invasiveness, and adherens junction formation in uveal melanoma cells.
Authors: Jannie KM, Stipp CS, Weiner JA
PLoS ONE, 2012-06-26;7(6):e39330.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Activated leukocyte cell adhesion molecule expression predicts lymph node metastasis in oral squamous cell carcinoma.
Authors: van den Brand M, Takes RP, Blokpoel-deRuyter M, Slootweg PJ, van Kempen LC
Oral Oncol., 2010-04-03;46(5):393-8.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr