Human alpha 1B-Glycoprotein Antibody

Catalog #: AF7757 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7757
AF7757-SP

Detection of Human alpha 1B-Glycoprotein by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human alpha 1B-Glycoprotein Antibody Summary

Species Reactivity

Human

Specificity

Detects human alpha 1B-Glycoprotein in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Human embryonic kidney cell line HEK293-derived recombinant human alpha 1B-Glycoprotein
Ala22-Ser495
Accession # EAW72575

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

10 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human alpha 1B-Glycoprotein by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and human liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human a 1B-Glycoprotein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7757) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for a 1B-Glycoprotein at approximately 70-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Human alpha 1B-Glycoprotein by Simple Western^TM. Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for a 1B-Glycoprotein at approximately 94 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human a 1B-Glycoprotein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7757) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha 1B-Glycoprotein

A1BG (alpha-1 B/beta-glycoprotein; also C44 [in rodent]) is a 68-80 kDa monomeric plasma glycoprotein member of the Immunoglobulin superfamily of molecules. It is expressed by multiple cell types, principally hepatocytes in response to circulating growth hormone. Initially thought to act as an MMP inhibitor, A1BG is now known to bind to CRISP3, a distant member of a family of venomous molecules. CRISP3 is widely expressed and possesses no toxic properties. But it may play a key role in fertilization, where it temporarily blocks the reaction of PMNs to sperm in the uterus, thus increasing sperm lifespan and the likelihood of fertilization. Within this context, A1BG may regulate CRISP3 availability. A1BG has also been investigated as a biomarker in various cancers. Mature human A1BG is 474 amino acids (aa) in length (aa 22-495) and contains five V-type Ig-like domains. There is one isoform variant that utilizes Met123 as an alternative start site. Full-length human A1BG (aa 22-495) shares only 44% aa sequence identity with mouse A1BG.

Entrez Gene IDs

1 (Human); 117586 (Mouse)

Alternate Names

A1B; A1BG; ABG; alpha 1BGlycoprotein; alpha 1B-Glycoprotein; Alpha-1-B glycoprotein;alpha1B-glycoprotein;Alpha-1B-glycoprotein;alpha-1-B-glycoprotein;DKFZp686F0970;epididymis secretory sperm binding protein Li 163pA;HEL-S-163pA; GAB