Human alpha -Synuclein Antibody

Catalog #: AF1338
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1338
AF1338-SP

Save on RUO reagents! Up to 30% off + FREE shipping. See Details.

Detection of Human alpha -Synuclein by Western Blot.

4 Images

Product Details

Citations (2)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human alpha -Synuclein Antibody Summary

Species Reactivity

Human

Specificity

Detects human alpha -Synuclein in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human (rh) beta -Synuclein and rh gamma -Synuclein is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human alpha -Synuclein
Met1-Ala140
Accession # P37840

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

5-15 µg/mL

Human malignant melanoma cell line

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human alpha -Synuclein Monoclonal Antibody (Catalog # MAB13384).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human alpha-Synuclein DuoSet ELISA Kit (Catalog # DY1338-05) for convenient development of a sandwich ELISA.

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human alpha -Synuclein by Western Blot. Western blot shows lysates of human brain (motor cortex) tissue, human brain (cerebellum) tissue, SH-SY5Y human neuroblastoma cell line, and U-87 MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human a-Synuclein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1338) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). A specific band was detected for a-Synuclein at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

View Larger

alpha -Synuclein in Human Brain. a-Synuclein was detected in immersion fixed paraffin-embedded sections of human brain (hypothalamus) using Goat Anti-Human a-Synuclein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1338) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

ELISA

View Larger

Human alpha -Synuclein ELISA Standard Curve. Recombinant Human a-Synuclein protein was serially diluted 2-fold and captured by Mouse Anti-Human a-Synuclein Monoclonal Antibody (MAB13384) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human a-Synuclein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1338) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Simple Western

View Larger

Detection of Human alpha -Synuclein by Simple Western^TM. Simple Western lane view shows lysates of SK‑Mel‑28 human malignant melanoma cell line, loaded at 0.2 mg/ml. A specific band was detected for alpha -Synuclein at approximately 21 kDa (as indicated) using 10 µg/mL of Goat Anti-Human alpha -Synuclein Antigen Affinity-purified Polyclonal Antibody (AF1338). This experiment was conducted under reducing conditions and using the kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha-Synuclein

alpha -Synuclein is a cytoplasmic protein that is predominantly expressed in the central nervous system. It is a minor protein found in amyloid deposition that is a hallmark of Alzheimer's disease. Defects in SNCA are associated with familial Parkinson's disease.

Entrez Gene IDs

6622 (Human); 20617 (Mouse); 29219 (Rat); 641350 (Porcine); 282857 (Bovine)

Alternate Names

alpha-Synuclein; I+/--synuclein; MGC110988; NACP; non A-beta component of AD amyloid; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; PARK1; PARK4; PD1; SNCA; synuclein alpha-140; synuclein, alpha (non A4 component of amyloid precursor); Synuclein-alpha; truncated alpha synuclein

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human alpha -Synuclein Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

Mitochondrial localization of alpha-synuclein protein in alpha-synuclein overexpressing cells.
Authors: Shavali S, Brown-Borg HM, Ebadi M, Porter J
Neurosci. Lett., 2008-05-08;439(2):125-8.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot
Gene expression changes in postmortem tissue from the rostral pons of multiple system atrophy patients.
Authors: Langerveld AJ, Mihalko D, DeLong C, Walburn J, Ide CF
Mov. Disord., 2007-04-30;22(6):766-77.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P