Human Apolipoprotein A-I/ApoA1 Antibody

Detection of Human Apolipoprotein A‑I/ ApoA1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and Hep3B human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human Apolipoprotein A-I/ApoA1 Monoclonal Antibody (Catalog # MAB36641) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Apolipoprotein A-I/ApoA1 at approximately 25-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Apolipoprotein A‑I/ApoA1 in HepG2 Human Cell Line by Flow Cytometry HepG2 human hepatocellular carcinoma cell line was stained with Rabbit Anti-Human Apolipoprotein A-I/ApoA1 Monoclonal Antibody (Catalog # MAB36641, filled histogram) or isotype control antibody (Catalog # MAB1050, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Apolipoprotein A‑I/ApoA1 in Human Hepatocytes. Apolipoprotein A-I/ApoA1 was detected in immersion fixed human hepatocytes using Rabbit Anti-Human Apolipoprotein A-I/ApoA1 Monoclonal Antibody (Catalog # MAB36641) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell secretion. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Apolipoprotein A‑I/ApoA1 in HepG2 Human Cell Line. Apolipoprotein A-I/ApoA1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Rabbit Anti-Human Apolipoprotein A-I/ApoA1 Monoclonal Antibody (Catalog # MAB36641) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Apolipoprotein A‑I/ApoA1 in Human Liver Cancer Tissue. Apolipoprotein A-I/ApoA1 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Rabbit Anti-Human Apolipoprotein A-I/ApoA1 Monoclonal Antibody (Catalog # MAB36641) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.