Human BMP-6 Antibody Summary
Gln382-His513
Accession # P22004
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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BMP‑6 in Human Lung. BMP-6 was detected in immersion fixed paraffin-embedded sections of human lung using Goat Anti-Human BMP-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF507) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to smooth muscle in vasculature. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Alkaline Phosphatase Production Induced by BMP‑6 and Neutralization by Human BMP‑6 Antibody. Recombinant Human BMP-6 (Catalog # 507-BP) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP-6 (0.15 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human BMP-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF507). The ND50 is typically 0.75-3.75 µg/ml in the presence of L-ascorbic acide (50 µg/mL).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BMP-6
Human BMP-6 is one of at least 15 structurally and functionally related BMPs, which are members of the transforming growth factor beta (TGF-beta ) superfamily. BMPs were originally identified as protein regulators of cartilage and bone formation. However, they have since been shown to be involved in embryogenesis and morphogenesis of various tissues and organs. BMPs have also been shown to regulate the growth, differentiation, chemotaxis and apoptosis of various cell types, including mesenchymal cells, epithelial cells, hematopoietic cells and neuronal cells. Similarly to other TGF-beta family proteins, BMPs are highly conserved across animal species. At the amino acid sequence level, mature human and mouse BMP-6 shares 96% amino acid sequence identity. BMP-6 is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxy-terminal domain. Biologically active BMP-6 is a disulfide-linked homodimer of the carboxy-terminal 132 amino acid residues that contains the characteristic seven conserved cysteine residues involved in the formation of the cysteine knot and the single interchain disulfide bond. Cellular responses to BMP-6 have been shown to be mediated by the formation of hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors. Based on amino acid sequence similarity, BMP-5, -6, -7 and -8 are in the same subgroup.
- Ebendal, T. et al. (1998) J. Neurosci. Res. 51:139.
- Reddi, A.H. (1998) Nature Biotechnology 16:247.
Product Datasheets
Citations for Human BMP-6 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Serum Proteases Potentiate BMP-Induced Cell Cycle Re-entry of Dedifferentiating Muscle Cells during Newt Limb Regeneration
Authors: I Wagner, H Wang, PM Weissert, WL Straube, A Shevchenko, M Gentzel, G Brito, A Tazaki, C Oliveira, T Sugiura, A Shevchenko, A Simon, DN Drechsel, EM Tanaka
Dev. Cell, 2017-03-27;40(6):608-617.e6.
Applications: Western Blot -
H3K9 methylation is a barrier during somatic cell reprogramming into iPSCs.
Authors: Chen, Jiekai, Liu, He, Liu, Jing, Qi, Jing, Wei, Bei, Yang, Jiaqi, Liang, Hanquan, Chen, You, Chen, Jing, Wu, Yaran, Guo, Lin, Zhu, Jieying, Zhao, Xiangjie, Peng, Tianran, Zhang, Yixin, Chen, Shen, Li, Xuejia, Li, Dongwei, Wang, Tao, Pei, Duanqing
Nat Genet, 2012-12-02;45(1):34-42.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
A rapid and sensitive bioassay for the simultaneous measurement of multiple bone morphogenetic proteins. Identification and quantification of BMP4, BMP6 and BMP9 in bovine and human serum.
Authors: Herrera B, Inman GJ
BMC Cell Biol., 2009-03-19;10(0):20.
Species: Human
Sample Types: Recombinant Protein
Applications: Neutralization -
Bone morphogenetic protein-6 promotes osteoblastic prostate cancer bone metastases through a dual mechanism.
Authors: Dai J, Keller J, Zhang J, Lu Y, Yao Z, Keller ET
Cancer Res., 2005-09-15;65(18):8274-85.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
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