Human BRIX Antibody

Catalog #: AF4024 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4024
AF4024-SP

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Detection of Human BRIX by Western Blot.

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All BRIX Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human BRIX Antibody Summary

Species Reactivity

Human

Specificity

Detects human BRIX in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human BRIX
Ser131-Lys353
Accession # Q8TDN6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human BRIX by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human BRIX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4024) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for BRIX at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BRIX

BRIX (Biogenesis of ribosomes in Xenopus; also BRIX domain-containing protein 2) is a 41 kDa (predicted) member of the BXDC2 family of proteins. It is found in nucleoli, and appears to participate in the formation of a complex associated with pre-rRNA and enzymes that generate 60S ribosomal subunits. Human BRIX is 353 amino acids (aa) in length. It is characterized by the presence of a BRIX domain that lies between aa 60‑249. There is one splice variant that shows a 17 aa substitution for aa 11‑27. Over aa 131‑353, human BRIX shares 93% aa identity with mouse BRIX.

Long Name

Brix Domain Containing 2

Entrez Gene IDs

55299 (Human); 67832 (Mouse); 294799 (Rat)

Alternate Names

brix domain containing 2; Brix domain-containing protein 2; BRIX; BRIX1; BRIXBXDC2; BRX1, biogenesis of ribosomes, homolog (S. cerevisiae); BXDC2; FLJ11100; ribosome biogenesis protein BRX1 homolog