Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Immunocytochemistry, ELISA (detection), ELISA Development, ELISA Development (Detection)

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all C1qR1/CD93 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human C1q R1/CD93
Ala24-Lys580
Accession # Q9NPY3

Specificity

Detects human C1q R1/CD93 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse C1q R1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human C1qR1/CD93 Antibody

C1qR1/CD93 in U937 Human Cell Line.

C1qR1/CD93 was detected in immersion fixed U937 human histiocytic lymphoma cell line using Goat Anti-Human C1qR1/CD93 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2379) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human C1qR1/CD93 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Human whole blood monocytes

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed U937 human histiocytic lymphoma cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Human C1q R1/CD93

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: C1qR1/CD93

C1q R1, also known as CD93, C1q R, and C1q RP, is a single chain glycoprotein expressed on cells of myeloid origin, endothelial cells, and hematopoietic progenitor cells. In vitro, this molecule may mediate the enhancement of phagocytosis upon interaction with soluble defense collagens (1, 2).

References

  1. Kim, T.S. et al. (2000) Mol. Immunol. 37:377.
  2. Dean, Y.D. and P. Gasque (2001) Eur. J. Immunol. 31:1370.

Long Name

Complement Component C1q Receptor

Alternate Names

AA4 Antigen, C1q R1, C1q Rp, C1qRp, CD93, Collectin Receptor, Ly68

Entrez Gene IDs

22918 (Human); 17064 (Mouse); 84398 (Rat)

Gene Symbol

CD93

UniProt

Q9NPY3

Additional C1qR1/CD93 Products

Product Documents for Human C1qR1/CD93 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human C1qR1/CD93 Antibody

For research use only

Citations for Human C1qR1/CD93 Antibody

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Protocols

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