Human Caspase-1 Antibody Summary
Asn120-Asp297
Accession # P29466
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Caspase‑1 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line and THP-1 human acute monocytic leukemia cell line. PVDF Membrane was probed with 0.25 µg/mL of Goat Anti-Human Caspase-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6215) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Caspase-1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
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Caspase‑1 in THP‑1 Human Cell Line. Caspase-1 was detected in immersion fixed THP-1 human acute monocytic leukemia cell line using Goat Anti-Human Caspase-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6215) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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Detection of Human Caspase‑1 by Simple WesternTM. Simple Western lane view shows lysates of A431 human epithelial carcinoma parental cell line and Caspase-1 knockout A431 cell line (KO), loaded at 0.2 mg/mL. A specific band was detected for Caspase-1 at approximately 52 kDa (as indicated) using 25 µg/mL of Goat Anti-Human Caspase-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6215) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Western Blot Shows Human Caspase‑1 Specificity by Using Knockout Cell Line. Western blot shows lysates of A431 human epithelial carcinoma parental cell line and Caspase-1 knockout A431 cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Caspase-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6215) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Caspase-1 at approximately 50 kDa (as indicated) in the parental A431 cell line, but is not detectable in knockout A431 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Caspase-1
Caspase-1, also known as IL-1 beta -converting enzyme (ICE), is an aspartic protease that plays a key role in the inflammatory response and apoptosis. Caspase-1 precursor (about 50kDa) can be cleaved and the active enzyme consists of a complex of two 20 kDa (aa 120-297) and two 10 kDa (aa 317-404) subunits which associate following cleavage of inactive precursors. Caspase-1 is required for proteolytic cleavage of the IL-1 beta precursor to form the active proinflammatory cytokine. Alternate splicing generates several additional Caspase-1 isoforms with deletions in the propeptide regions or also in the mature subunits. Within the large subunit, human Caspase 1 shares 61% aa sequence identity with mouse and rat Caspase-1.
Product Datasheets
Citations for Human Caspase-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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NF?B and NLRP3/NLRC4 inflammasomes regulate differentiation, activation and functional properties of monocytes in response to distinct SARS-CoV-2 proteins
Authors: Tsukalov, I;Sánchez-Cerrillo, I;Rajas, O;Avalos, E;Iturricastillo, G;Esparcia, L;Buzón, MJ;Genescà, M;Scagnetti, C;Popova, O;Martin-Cófreces, N;Calvet-Mirabent, M;Marcos-Jimenez, A;Martínez-Fleta, P;Delgado-Arévalo, C;de Los Santos, I;Muñoz-Calleja, C;Calzada, MJ;González Álvaro, I;Palacios-Calvo, J;Alfranca, A;Ancochea, J;Sánchez-Madrid, F;Martin-Gayo, E;
Nature communications
Species: Human
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
A Salmonella Typhi RNA thermosensor regulates virulence factors and innate immune evasion in response to host temperature
Authors: SM Brewer, C Twittenhof, J Kortmann, SW Brubaker, J Honeycutt, LM Massis, THM Pham, F Narberhaus, DM Monack
PloS Pathogens, 2021-03-02;17(3):e1009345.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Human metapneumovirus activates NOD-like receptor protein 3 inflammasome via its small hydrophobic protein which plays a detrimental role during infection in mice
Authors: VB Lê, J Dubois, C Couture, MH Cavanagh, O Uyar, A Pizzorno, M Rosa-Calat, MÈ Hamelin, G Boivin
PLoS Pathog., 2019-04-09;15(4):e1007689.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Cutting Edge: Inflammasome Activation in Primary Human Macrophages Is Dependent on Flagellin.
Authors: Kortmann J, Brubaker S, Monack D
J Immunol, 2015-06-24;195(3):815-9.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Staphylococcus aureus activates the NLRP3 inflammasome in human and rat conjunctival goblet cells.
Authors: McGilligan V, Gregory-Ksander M, Li D, Moore J, Hodges R, Gilmore M, Moore T, Dartt D
PLoS ONE, 2013-09-10;8(9):e74010.
Species: Human, Rat
Sample Types: Cell Lysates
Applications: Western Blot
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