Human Cathepsin O Antibody Summary
Species Reactivity
Human
Specificity
Detects human Cathepsin O in direct ELISAs and Western blots. In Western blots, less than 2% cross‑reactivity with recombinant human Cathepsin A, B, C, D, L, and Z is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cathepsin O
Asp24-Val321
Accession # P43234
Asp24-Val321
Accession # P43234
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated
Applications
Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Cathepsin O
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human breast cancer tissue
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Cathepsin O, see our available Western blot detection antibodies
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Reconstitution Calculator
Preparation and Storage
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cathepsin O
Entrez Gene IDs
1519 (Human)
Alternate Names
Cathepsin O; CTSO; CTSO1; EC 3.4.22.42
Product Datasheets
FAQs
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