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Human CCL20/MIP-3 alpha Antibody

Catalog #: AF360
23 Citations Datasheet / COA / SDS
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AF360
AF360-SP
CCL20/MIP‑3 alpha in Human PBMCs.
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Citations (23)
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Human CCL20/MIP-3 alpha Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCL20/MIP-3 alpha in direct ELISAs.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human CCL20/MIP-3 alpha
Ala27-Met96 (Asn95Asp)
Accession # P78556
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
3-15 µg/mL
Immersion fixed paraffin-embedded sections of human skin and human testis
Immunocytochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize CCL20/MIP‑3 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR6. The Neutralization Dose (ND50) is typically 0.2-1.0 µg/mL in the presence of 10 ng/mL Recombinant Human CCL20/MIP‑3 alpha.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry CCL20/MIP‑3a antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

CCL20/MIP‑3 alpha in Human PBMCs. CCL20/MIP-3a was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stumulated with LPS using Human CCL20/MIP-3a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF360) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry CCL20/MIP‑3a antibody in Human Skin by Immunohistochemistry (IHC-P). View Larger

CCL20/MIP‑3 alpha in Human Skin. CCL20/MIP‑3a was detected in immersion fixed paraffin-embedded sections of human skin using Goat Anti-Human CCL20/MIP‑3a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF360) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Neutralization Chemotaxis Induced by CCL20/MIP‑3 alpha and Neutralization by Human CCL20/MIP‑3 alpha Antibody. View Larger

Chemotaxis Induced by CCL20/MIP‑3 alpha and Neutralization by Human CCL20/MIP‑3 alpha Antibody. Recombinant Human CCL20/MIP‑3a (360-MP) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (AR002). Chemotaxis elicited by Recombinant Human CCL20/MIP‑3a (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL20/MIP‑3a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF360). The ND50 is typically 0.2-1.0 µg/mL.

Immunohistochemistry View Larger

Detection of CCL20/MIP‑3 alpha in Human Testis. CCL20/MIP‑3 alpha was detected in immersion fixed paraffin-embedded sections of Human Testis using Goat Anti-Human CCL20/MIP‑3 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF360) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to myoid cells, spermatogonia and spermatids.. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL20/MIP-3 alpha

CCL20, also known as LARC (Liver and Activation-regulated Chemokine) and as Exodus, is one of many novel beta chemokines identified through bioinformatics. CCL20 cDNA encodes a 96 amino acid (aa) residue precursor protein with a 26 aa residue signal peptide that is predicted to be cleaved to form the 70 aa residue mature secreted protein. CCL20 is distantly related to other beta chemokines (20 - 28% aa sequence identity) and the gene for CCL20 has been mapped to chromosome 2 rather than 17.

CCL20 has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. The expression of CCL20 is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL-10. Synthetic or recombinant CCL20 has been shown to be chemotactic for lymphocytes and to inhibit proliferation of myeloid progenitors in colony formation assays. CCL20 has now been shown to be a unique functional ligand for CCR-6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34+ cord blood precursors.

References
  1. Baba, M. et al. (1997) J. Biol. Chem. 272:14893.
  2. Hromas, R. et al. (1997) Blood 89:3315.
  3. Greaves, D.R. et al. (1997) J. Exp. Med. 186:837.
Entrez Gene IDs
6364 (Human); 20297 (Mouse); 29538 (Rat)
Alternate Names
beta chemokine exodus-1; Beta-chemokine exodus-1; CC chemokine LARC; C-C motif chemokine 20; CCL20; chemokine (C-C motif) ligand 20; CKb4; exodus-1; LARC; LARCLiver and activation-regulated chemokine; MIP3 alpha; MIP-3 alpha; MIP-3a; MIP-3-alpha; MIP3AMacrophage inflammatory protein 3 alpha; SCYA20Small-inducible cytokine A20; small inducible cytokine subfamily A (Cys-Cys), member 20; ST38

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Citations for Human CCL20/MIP-3 alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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  1. Tumor-associated neutrophils activated by tumor-derived CCL20 (C-C motif chemokine ligand 20) promote T cell immunosuppression via programmed death-ligand 1 (PD-L1) in breast cancer
    Authors: Louis Boafo Kwantwi, Shujing Wang, Wenjun Zhang, Weidong Peng, Zeyu Cai, Youjing Sheng et al.
    Bioengineered
  2. miR-21 and its target gene CCL20 are both highly overexpressed in the microenvironment of colorectal tumors: Significance of their regulation
    Authors: BENJAMIN VICINUS, CLAUDIA RUBIE, NATHALIE STEGMAIER, VILMA OLIVEIRA FRICK, KATHRIN KÖLSCH, ANNE KAUFFELS et al.
    Oncology Reports
  3. Human Papillomavirus Type 8 Interferes with a Novel C/EBPβ-Mediated Mechanism of Keratinocyte CCL20 Chemokine Expression and Langerhans Cell Migration.
    Authors: Sperling T, Oldak M, Walch-Ruckheim B, Wickenhauser C, Doorbar J, Pfister H, Malejczyk M, Majewski S, Keates AC, Smola S
    PLoS Pathog., 2012-07-26;8(7):e1002833.
  4. Single cell transcriptomic analysis of human amnion identifies cell-specific signatures associated with membrane rupture and parturition
    Authors: Wang W, Lin Y, Zhang F et al.
    Cell Biosci.
  5. Regulation of the Psoriatic Chemokine CCL20 by E3 Ligases Trim32 and Piasy in Keratinocytes
    Authors: Yuangang Liu, James P. Lagowski, Shangpu Gao, James H. Raymond, Clifton R. White, Molly F. Kulesz-Martin
    Journal of Investigative Dermatology
  6. Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells
    Authors: F Parween, SP Singh, HH Zhang, N Kathuria, FA Otaizo-Car, A Shamsaddin, PJ Gardina, S Ganesan, J Kabat, HA Lorenzi, TG Myers, JM Farber
    bioRxiv : the preprint server for biology, 2023-02-13;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. An E. coli display method for characterization of peptide-sensor kinase interactions
    Authors: KR Brink, MG Hunt, AM Mu, K Groszman, KV Hoang, KP Lorch, BH Pogostin, JS Gunn, JJ Tabor
    Nature Chemical Biology, 2022-12-08;0(0):.
    Species: Human
    Sample Types: Cell Lysates
  8. Gene expression profiling of primary human type I alveolar epithelial cells exposed to Bacillus anthracis spores reveals induction of neutrophil and monocyte chemokines
    Authors: JL Booth, ES Duggan, VI Patel, W Wu, DM Burian, DC Hutchings, VL White, KM Coggeshall, MG Dozmorov, JP Metcalf
    Microb. Pathog., 2018-04-25;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Neutralization
  9. Role of CCL20/CCR6 and the ERK signaling pathway in lung adenocarcinoma
    Authors: XP Zhang, ZJ Hu, AH Meng, GC Duan, QT Zhao, J Yang
    Oncol Lett, 2017-10-23;14(6):8183-8189.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  10. An initial investigation into endothelial CC chemokine expression in the human rheumatoid synovium.
    Authors: Rump L, Mattey D, Kehoe O, Middleton J
    Cytokine, 2017-09-01;97(0):133-140.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  11. Fusobacterium nucleatum subspecies animalis influences pro-inflammatory cytokine expression and monocyte activation in human colorectal tumors
    Authors: X Ye, R Wang, R Bhattachar, D Boulbes, F Fan, L Xia, H Adoni, NJ Ajami, MC Wong, DP Smith, JF Petrosino, S Venable, W Qiao, V Baladanday, DM Maru, LM Ellis
    Cancer Prev Res (Phila), 2017-05-08;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Transforming Growth Factor-beta and Interleukin-1beta Signaling Pathways Converge on the Chemokine CCL20 Promoter.
    Authors: Brand O, Somanath S, Moermans C, Yanagisawa H, Hashimoto M, Cambier S, Markovics J, Bondesson A, Hill A, Jablons D, Wolters P, Lou J, Marks J, Baron J, Nishimura S
    J Biol Chem, 2015-04-27;290(23):14717-28.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  13. Preferential recruitment of Th17 cells to cervical cancer via CCR6-CCL20 pathway.
    Authors: Yu, Qing, Lou, Xiang-mi, He, Yan
    PLoS ONE, 2015-03-13;10(3):e0120855.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Bioassay
  14. Microarray analysis of gene expression by microdissected epidermis and dermis in mycosis fungoides and adult T-cell leukemia/lymphoma.
    Authors: Hashikawa K, Yasumoto S, Nakashima K, Arakawa F, Kiyasu J, Kimura Y, Saruta H, Nakama T, Yasuda K, Tashiro K, Kuhara S, Hashimoto T, Ohshima K
    Int J Oncol, 2014-06-26;45(3):1200-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  15. CCR6, the sole receptor for the chemokine CCL20, promotes spontaneous intestinal tumorigenesis.
    Authors: Nandi, Bisweswa, Pai, Christin, Huang, Qin, Prabhala, Rao H, Munshi, Nikhil C, Gold, Jason S
    PLoS ONE, 2014-05-27;9(5):e97566.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  16. IL-22 negatively regulates Helicobacter pylori-induced CCL20 expression in gastric epithelial cells.
    Authors: Chen J, Wu M, Kuo S, Liao F
    PLoS ONE, 2014-05-13;9(5):e97350.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  17. EBV infection is common in gingival epithelial cells of the periodontium and worsens during chronic periodontitis.
    Authors: Vincent-Bugnas S, Vitale S, Mouline C, Khaali W, Charbit Y, Mahler P, Precheur I, Hofman P, Maryanski J, Doglio A
    PLoS ONE, 2013-12-19;8(12):e80336.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  18. Lung myeloid dendritic cells coordinately induce TH1 and TH17 responses in human emphysema.
    Authors: Shan M, Cheng HF, Song LZ
    Sci Transl Med, 2009-10-28;1(4):4ra10.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-Fr
  19. Macrophage inflammatory protein-3alpha is a novel serum marker for nasopharyngeal carcinoma detection and prediction of treatment outcomes.
    Authors: Chang KP, Hao SP, Chang JH, Wu CC, Tsang NM, Lee YS, Hsu CL, Ueng SH, Liu SC, Liu YL, Wei PC, Liang Y, Chang YS, Yu JS
    Clin. Cancer Res., 2008-11-01;14(21):6979-87.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  20. Substance P regulates macrophage inflammatory protein 3alpha/chemokine C-C ligand 20 (CCL20) with heme oxygenase-1 in human periodontal ligament cells.
    Authors: Lee SK, Pi SH, Kim SH, Min KS, Lee HJ, Chang HS, Kang KH, Kim HR, Shin HI, Lee SK, Kim EC
    Clin. Exp. Immunol., 2007-10-09;150(3):567-75.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  21. Expression and role of CCR6/CCL20 chemokine axis in pulmonary sarcoidosis.
    Authors: Facco M, Baesso I, Miorin M, Bortoli M, Cabrelle A, Boscaro E, Gurrieri C, Trentin L, Zambello R, Calabrese F, Cassatella MA, Semenzato G, Agostini C
    J. Leukoc. Biol., 2007-07-05;82(4):946-55.
    Species: Human
    Sample Types: BALF, Whole Tissue
    Applications: ELISA Development, IHC-Fr, IHC-P
  22. Cutting edge: nonproliferating mature immune cells form a novel type of organized lymphoid structure in idiopathic pulmonary fibrosis.
    Authors: Marchal-Somme J, Uzunhan Y, Marchand-Adam S, Valeyre D, Soumelis V, Crestani B, Soler P
    J. Immunol., 2006-05-15;176(10):5735-9.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  23. Sequential involvement of CCR2 and CCR6 ligands for immature dendritic cell recruitment: possible role at inflamed epithelial surfaces.
    Authors: Vanbervliet B, Homey B, Durand I, Massacrier C, Ait-Yahia S, de Bouteiller O, Vicari A, Caux C
    Eur J Immunol, 2002-01-01;32(1):231-42.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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