Human CCL25/TECK Antibody Summary
Gln24-Leu150
Accession # AAB69981
Applications
Measured by its ability to neutralize CCL25/TECK-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR9. The Neutralization Dose (ND50) is typically 5.00 - 40.0 µg/mL in the presence of 1.50 µg/mL Recombinant Human CCL25/TECK.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Chemotaxis Induced by CCL25/TECK and Neutralization by Human CCL25/TECK Antibody. Recombinant Human CCL25/TECK chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR9 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Human CCL25/TECK (1.5 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL25/TECK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF334). The Neutralization Dose (ND50) is typically 5.00 - 40.0 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL25/TECK
CCL25 (thymus-expressed chemokine) is a novel CC chemokine that is distantly related (approximately 20% amino acid sequence identity) to other CC chemokines. Human CCL25 cDNA encodes a 150 amino acid residue precursor protein with a 23 amino acid residue signal peptide that is cleaved to yield a 127 residue mature protein. Mouse CCL25 cDNA has also been cloned and shown to encode a 144 amino acid protein that exhibits 49% amino acid sequence identity to human CCL25. The expresssion of human and mouse CCL25 was shown to be highly restricted to the thymus and small intestine. Although dendritic cells have been demonstrated to be the source of CCL25 production in the thymus, dendritic cells derived from bone marrow do not express CCL25. The gene for mouse CCL25 has been mapped to chromosome 8 rather than chromosome 11 where many mouse CC chemokines are clustered. Recombinant human and mouse CCL25 have been shown to be chemotactic for activated macrophages, dendritic cells and thymocytes. CCL25 has also been shown to be a specific agonist for the orphan receptor called GPR-9-6, now named CCR9.
Product Datasheets
Citation for Human CCL25/TECK Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Intestinal CCL25 expression is increased in colitis and correlates with inflammatory activity
Authors: Palak J. Trivedi, Tony Bruns, Stephen Ward, Martina Mai, Carsten Schmidt, Gideon M. Hirschfield et al.
Journal of Autoimmunity
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