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Human CD177 Antibody

Catalog #: AF3505
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3505
AF3505-SP
Detection of CD177 in Human Lymphocytes by Flow Cytometry.
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Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD177 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD177 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD177
Leu22-Gly407
Accession # AAH29167
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CD177 (Catalog # 3505-CD)
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD177 antibody in Human Lymphocytes antibody by Flow Cytometry. View Larger

Detection of CD177 in Human Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Goat Anti-Human CD177 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3505, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD177

CD177 is a polymorphic gene in the leukocyte antigen (Ly6) uPAR superfamily. It encodes at least two alleles of the neutrophilic membrane protein PRV1 (Polycythemic Rubra Vera protein 1) and NB1 (also named HNA-2a) that are glycosyl-phosphotidylinositol (GP1) anchored proteins of 58‑64 kDa. CD177 has two highly homologous cysteine-rich uPAR/Ly6 domains. The mRNA levels of CD177 is elevated under conditions where neutrophil counts are increased and is a useful marker for polycythemic vera. Mature human and mouse CD177 share 43% amino acid sequence identity. CD177 is expressed on the surface of neutrophils through a glycosylphosphatidylinositol (GPI) anchor (1-3).

References
  1. Stroncek, D.F. (2007) Curr. Opin. Hematol. 14:688.
  2. Kissel, K. et al. (2001) Eur. J. Immunol. 31:1301.
  3. Temerinac, S. et al. (2000) Blood 95:2569.
Long Name
Polycythemia Rubra Vera Protein 1
Entrez Gene IDs
57126 (Human); 68891 (Mouse)
Alternate Names
CD177 antigenNB1 GP; CD177 molecule; CD177; HNA-2a; HNA2Acell surface receptor; Human neutrophil alloantigen 2a; NB1 GP; NB1PRV-1; polycythemia rubra vera 1; Polycythemia rubra vera protein 1; PRV1; PRV1NB1 glycoprotein

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Citation for Human CD177 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Complement Receptor Mac-1 Is an Adaptor for NB1 (CD177)-mediated PR3-ANCA Neutrophil Activation.
    Authors: Jerke U, Rolle S, Dittmar G, Bayat B, Santoso S, Sporbert A, Luft F, Kettritz R
    J. Biol. Chem., 2010-12-30;286(9):7070-81.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot

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