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Human CD4 Antibody

Catalog #: AF-379-NA
29 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF-379-NA
AF-379-SP
CD4 in Human T Cells.
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Product Details
Citations (29)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD4 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD4
Lys26-Trp390
Accession # P01730
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
PBMC and SUP‑T1 human T cell lymphoblastic lymphoma cells
Simple Western
10 µg/mL
Human PBMC and SUP‑T1 human T cell lymphoblastic lymphoma cell line
Immunohistochemistry
1-15 µg/mL
Immersion fixed paraffin-embedded sections of human tonsil tissue
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry CD4 antibody in Human T Cells by Immunocytochemistry (ICC). View Larger

CD4 in Human T Cells. CD4 was detected in immersion fixed human T cells using 2 µg/mL Goat Anti-Human CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑379‑NA) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry CD4 antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

CD4 in Human PBMCs. CD4 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-379-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry CD4 antibody in Human Tonsil Tissue by Immunohistochemistry (IHC-P). View Larger

CD4 in Human Tonsil Tissue. CD4 was detected in immersion fixed paraffin-embedded sections of human tonsil tissue using Goat Anti-Human CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-379-NA) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface of lymphocytes. Staining was performed using our IHC Staining with VisUCyte HRP Polymer Detection Reagents protocol.

Western Blot View Larger

Detection of Human CD4 by Western Blot. Western blot shows lysates of PBMC and SUP‑T1 human T cell lymphoblastic lymphoma cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-379-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD4 at approximately ~55kDa kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Simple Western View Larger

Detection of Human CD4 by Simple WesternTM. Simple Western lane view shows lysates of Human PBMC and SUP‑T1 human T cell lymphoblastic lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for CD4 at approximately 65 kDa (as indicated) using 10 µg/mL of Goat Anti-Human CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-379-NA). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD4

CD4 is an approximately 55 kDa type I membrane glycoprotein that is expressed predominantly on most thymocytes and a subset of mature T lymphocytes. In humans, CD4 is also expressed to a lesser extent on monocytes and macrophage related cells. Human CD4 cDNA encodes a 458 amino acid (aa) residue precursor protein with a 25 aa residue signal peptide, a 371 aa residue extracellular region containing four immunoglobulin homology domains, a 24 aa residue transmembrane domain and a 38 aa residue cytoplasmic domain. CD4 is a coreceptor required for T cell recognition of antigens that are presented by class II major histocompatibility complexes. CD4 has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.

References
  1. Capon, D.I. et al. (1991) Annu. Rev. Immunol. 9:649.
Entrez Gene IDs
920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 101864991 (Cynomolgus Monkey); 493775 (Feline)
Alternate Names
CD_antigen: CD4; CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

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Citations for Human CD4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

29 Citations: Showing 1 - 10
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  1. Human Inner Ear Immune Activity: A Super-Resolution Immunohistochemistry Study
    Authors: Liu W, Kampfe Nordstrom C, Danckwardt-Lilliestrom N, Rask-Andersen H,
    Front Neurol
  2. Stem-like T cells are associated with the pathogenesis of ulcerative colitis in humans
    Authors: Li, Y;Ramírez-Suástegui, C;Harris, R;Castañeda-Castro, FE;Ascui, G;Pérez-Jeldres, T;Diaz, A;Morong, C;Giles, DA;Chai, J;Seumois, G;Sanchez-Elsner, T;Cummings, F;Kronenberg, M;Vijayanand, P;
    Nature immunology
    Species: Human
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  3. Tertiary lymphoid structures correlate with enhancement of antitumor immunity in esophageal squamous cell carcinoma
    Authors: Nakamura, S;Ohuchida, K;Hayashi, M;Katayama, N;Tsutsumi, C;Yamada, Y;Hisano, K;Okuda, S;Ohtsubo, Y;Iwamoto, C;Torata, N;Mizuuchi, Y;Shindo, K;Nakata, K;Moriyama, T;Morisaki, T;Oda, Y;Nakamura, M;
    British journal of cancer
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. An agonistic anti-signal regulatory protein alpha antibody for chronic inflammatory diseases
    Authors: Markus M. Xie, Bingbing Dai, Jason A. Hackney, Tianhe Sun, Juan Zhang, Janet K. Jackman et al.
    Cell Reports Medicine
  5. Establishment of a humanized mouse model of keloid diseases following the migration of patient immune cells to the lesion: Patient-derived keloid xenograft (PDKX) model
    Authors: Lee, AR;Lee, SY;Choi, JW;Um, IG;Na, HS;Lee, JH;Cho, ML;
    Experimental & molecular medicine
  6. Step-dose IL-7 treatment promotes systemic expansion of T cells and alters immune cell landscape in blood and lymph nodes
    Authors: Hrishikesh Pandit, Antonio Valentin, Matthew Angel, Claire Deleage, Cristina Bergamaschi, Jenifer Bear et al.
    iScience
  7. Tumor Lymphocyte Infiltration Is Correlated with a Favorable Tumor Regression Grade after Neoadjuvant Treatment for Esophageal Adenocarcinoma
    Authors: R Haddad, O Zlotnik, T Goshen-Lag, M Levi, E Brook, B Brenner, Y Kundel, I Ben-Aharon, H Kashtan
    Journal of personalized medicine, 2022-04-13;12(4):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  8. Microbial Protein Binding to gC1qR Drives PLA2G1B-Induced CD4 T-Cell Anergy
    Authors: Julien Pothlichet, Annalisa Meola, Florence Bugault, Louise Jeammet, Anne G. Savitt, Berhane Ghebrehiwet et al.
    Frontiers in Immunology
  9. E4orf1 Suppresses E1B-Deleted Adenovirus Vaccine-Induced Immune Responses
    Authors: K Sangare, S Helmold Ha, M Moore, C Hogge, T Hoang, MA Rahman, DJ Venzon, C LaBranche, D Montefiori, M Robert-Gur, MA Thomas
    Vaccines, 2022-02-15;10(2):.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cyomtetry, Western Blot
  10. Distribution of Immune Cells Including Macrophages in the Human Cochlea
    Authors: Liu W, Danckwardt-Lilliestr�m N, Schrott-Fischer A et al.
    Frontiers in Neurology
  11. Pilot Study of Anti-Th2 Immunotherapy for the Treatment of Breast Cancer-Related Upper Extremity Lymphedema
    Authors: BJ Mehrara, HJ Park, RP Kataru, J Bromberg, M Coriddi, JE Baik, J Shin, C Li, MR Cavalli, EM Encarnacio, M Lee, KJ Van Zee, E Riedel, JH Dayan
    Biology, 2021-09-18;10(9):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  12. Increased Circulating T Follicular Helper Cells Induced via IL-12/21 in Patients With Acute on Chronic Hepatitis B Liver Failure
    Authors: Bingying Du, Jiaming Teng, Rongkun Yin, Yuanyuan Tian, Tingwang Jiang, Yanan Du et al.
    Frontiers in Immunology
  13. PLA2G1B is involved in CD4 anergy and CD4 lymphopenia in HIV-infected patients
    Authors: Julien Pothlichet, Thierry Rose, Florence Bugault, Louise Jeammet, Annalisa Meola, Ahmed Haouz et al.
    Journal of Clinical Investigation
  14. Lymphoid Aggregates in the CNS of Progressive Multiple Sclerosis Patients Lack Regulatory T Cells
    Authors: Luisa Bell, Alexander Lenhart, Andreas Rosenwald, Camelia M. Monoranu, Friederike Berberich-Siebelt
    Frontiers in Immunology
  15. Persistence of a T Cell Infiltrate in Human Ganglia Years After Herpes Zoster and During Post-herpetic Neuralgia
    Authors: JP Sutherland, M Steain, ME Buckland, M Rodriguez, AL Cunningham, B Slobedman, A Abendroth
    Front Microbiol, 2019-09-11;10(0):2117.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  16. Cellular and inter-cellular rewiring of the human colon during ulcerative colitis
    Authors: Christopher S. Smillie, Moshe Biton, Jose Ordovas-Montanes, Keri M. Sullivan, Grace Burgin, Daniel B. Graham et al.
    Cell
  17. Distinct Inflammatory Profiles in Atopic and Nonatopic Patients With Chronic Rhinosinustis Accompanied by Nasal Polyps in Western China
    Authors: Luo Ba, Jintao Du, Feng Liu, Fenglin Yang, Miaomiao Han, Sixi Liu et al.
    Allergy, Asthma & Immunology Research
  18. Tumor-specific cytotoxic T lymphocyte activity determines colorectal cancer patient prognosis.
    Authors: Reissfelder C, Stamova S, Gossmann C, Braun M, Bonertz A, Walliczek U, Grimm M, Rahbari N, Koch M, Saadati M, Benner A, Buchler M, Jager D, Halama N, Khazaie K, Weitz J, Beckhove P
    J Clin Invest, 2014-12-22;125(2):739-51.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  19. HIV-1 gp120 activates the STAT3/interleukin-6 axis in primary human monocyte-derived dendritic cells.
    Authors: Del Corno M, Donninelli G, Varano B, Da Sacco L, Masotti A, Gessani S
    J Virol, 2014-07-09;88(19):11045-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  20. Slow turnover of HIV-1 receptors on quiescent CD4+ T cells causes prolonged surface retention of gp120 immune complexes in vivo.
    Authors: Suzuki, Yasuhiro, Gatanaga, Hiroyuki, Tachikawa, Natsuo, Oka, Shinichi
    PLoS ONE, 2014-02-06;9(2):e86479.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  21. A role for microRNA-155 modulation in the anti-HIV-1 effects of Toll-like receptor 3 stimulation in macrophages.
    Authors: Swaminathan G, Rossi F, Sierra L, Gupta A, Navas-Martin S, Martin-Garcia J
    PLoS Pathog, 2012-09-20;8(9):e1002937.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  22. Patients with cystic fibrosis have inducible IL-17+IL-22+ memory cells in lung draining lymph nodes.
    Authors: Chan Y, Chen K, Duncan S, Lathrop K, Latoche J, Logar A, Pociask D, Wahlberg B, Ray P, Ray A, Pilewski J, Kolls J
    J Allergy Clin Immunol, 2012-07-11;131(4):1117-29, 1129.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  23. Lymphoid Tissue Damage in HIV-1 Infection Depletes Naïve T Cells and Limits T Cell Reconstitution after Antiretroviral Therapy
    Authors: Ming Zeng, Peter J. Southern, Cavan S. Reilly, Greg J. Beilman, Jeffrey G. Chipman, Timothy W. Schacker et al.
    PLoS Pathogens
  24. T cell infiltrates in the muscles of patients with dermatomyositis and polymyositis are dominated by CD28null T cells.
    Authors: Fasth AE, Dastmalchi M, Rahbar A, Salomonsson S, Pandya JM, Lindroos E, Nennesmo I, Malmberg KJ, Soderberg-Naucler C, Trollmo C, Lundberg IE, Malmstrom V
    J. Immunol., 2009-09-14;183(7):4792-9.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  25. Restricted T cell receptor BV gene usage in the lungs and muscles of patients with idiopathic inflammatory myopathies.
    Authors: Englund P, Wahlstrom J, Fathi M, Rasmussen E, Grunewald J, Tornling G, Lundberg IE
    Arthritis Rheum., 2007-01-01;56(1):372-83.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  26. An effective cancer vaccine modality: lentiviral modification of dendritic cells expressing multiple cancer-specific antigens.
    Authors: Wang B, He J, Liu C, Chang LJ
    Vaccine, 2006-02-28;24(17):3477-89.
    Species: Human, Primate
    Sample Types: Whole Cells
    Applications: ICC
  27. Roles for herpes simplex virus type 1 UL34 and US3 proteins in disrupting the nuclear lamina during herpes simplex virus type 1 egress.
    Authors: Bjerke SL, Roller RJ
    Virology, 2006-01-19;347(2):261-76.
    Species: Human, Primate - Cholrocebus pygerythrus (Vervet Monkey)
    Sample Types: Whole Cells
    Applications: ICC
  28. A Map of Human Type 1 Diabetes Progression by Imaging Mass Cytometry
    Authors: Damond N, Engler S, Zanotelli VRT et al.
    Cell Metab.
  29. Integrative proteo-transcriptomic and immunophenotyping signatures of HIV-1 elite control phenotype: A cross-talk between glycolysis and HIF signaling
    Authors: AkusjArvi SS, Ambikan AT, Krishnan S Et al.
    iScience

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Human CD4 Antibody
By Anonymous on 04/06/2021
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Colon cancer tissue Species: Human
Immunocytochemistry/Immunofluorescence Human CD4 Antibody AF-379-NA
Human CD4 Antibody
By Anonymous on 12/02/2019
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Tonsil tissue Species: Human and Human tonsil
Immunocytochemistry/Immunofluorescence Human CD4 Antibody AF-379-NA