Human CEACAM-4 Antibody

Catalog #: MAB7845 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7845
MAB7845-SP

Detection of CEACAM‑4 in THP‑1 Human Cell Line by Flow Cytometry.

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All CEACAM-4 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CEACAM-4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CEACAM-4 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 822608

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human CEACAM-4
Phe36-Val140
Accession # O75871

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CEACAM‑4 in THP‑1 Human Cell Line by Flow Cytometry. THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human CEACAM-4 Monoclonal Antibody (Catalog # MAB7845, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CEACAM-4

CEACAMs are intercellular adhesion molecules that form Ca²⁺-dependent homo- and heterotypic interactions, and typically serve as pathogen receptors. All CEACAMs are heavily glycosylated and contain one N-terminal V-type Ig-like domain. CEACAMs also exhibit variability in their structure by containing transmembrane domains (#CEACAM-1, 3 & 4), GPI-linked domains (#5, 6, 7, & 8), or CD66 designations (#1, 3, 5, 6, & 7).

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 4

Entrez Gene IDs

1089 (Human)

Alternate Names

carcinoembryonic antigen CGM7; carcinoembryonic antigen gene family member 7; carcinoembryonic antigen-related cell adhesion molecule 4; CEACAM4; CEACAM-4; CGM7; CGM7_HUMAN