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Human Chorionic Gonadotropin alpha Chain ( alpha HCG) Antibody

Catalog #: MAB4169 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB4169
MAB4169-SP
Chorionic Gonadotropin, alpha Chain ( alpha HCG) in Human Placenta.
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Chorionic Gonadotropin alpha Chain ( alpha HCG) Antibody Summary

Species Reactivity
Human
Specificity
Detects human Chorionic Gonadotropin, alpha Chain ( alpha HCG) in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human FSH  beta or recombinant rat FSH  beta is observed.
Source
Monoclonal Mouse IgG2A Clone # 381012
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Chorionic Gonadotropin, alpha Chain ( alpha HCG)
Ala25-Ser116
Accession # P01215
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Chorionic Gonadotropin, alpha  Chain ( alpha  HCG)
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Chorionic Gonadotropin, a Chain (a HCG) antibody in Human Placenta by Immunohistochemistry (IHC-P). View Larger

Chorionic Gonadotropin, alpha Chain ( alpha HCG) in Human Placenta. Chorionic Gonadotropin, a Chain (a HCG) was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human Chorionic Gonadotropin, a Chain (a HCG) Monoclonal Antibody (Catalog # MAB4169) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chorionic Gonadotropin alpha Chain (HCG alpha)

Human Chorionic Gonadotropin, alpha chain (CGA) is a 92 aa glycopeptide that functions as the shared alpha subunit of the heterodimeric peptide hormones choriogonadotropin, leutinizing hormone, thryoid stimulating hormone, and follicle stimulating hormone. CGA circulates as a free molecule and in noncovalent complexes with the unique beta subunits of those hormones. CGA is secreted by the pituitary and placenta. Mature human CGA shares 69%‑73% aa sequence identity with canine, equine, feline, mouse, porcine, and rat CGA.

Entrez Gene IDs
1081 (Human)
Alternate Names
alpha HCG; CGA glycoprotein hormones, alpha polypeptide; CGA; CG-ALPHA; Chorionic Gonadotropin alpha Chain; FSHA; GPHa; GPHa1; HCG; LHA; TSHA

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Reviews for Human Chorionic Gonadotropin alpha Chain ( alpha HCG) Antibody

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Human Chorionic Gonadotropin alpha Chain ( alpha HCG) Antibody
By Anonymous on 05/03/2022
Application: IHC Sample Tested: Placental tissue Species: Human

incubation 15 µg/mL overnight at 4 °C

Immunohistochemistry Human Chorionic Gonadotropin alpha Chain ( alpha HCG) Antibody MAB4169
Human Chorionic Gonadotropin alpha Chain ( alpha HCG) Antibody
By Anonymous on 12/15/2016
Application: Immunocytochemistry/Immunofluorescence Sample Tested: embryonic stem cells,induced pluripotent stem cells Species: Human

We use the monoclonal antibody to detect human Chorionic Gonadotropin, alpha (CGA) Chain expressed in human embryonic stem cells and induced pluripotent stem cells (PSC) derived model for studying human placental trophoblasts in vitro. This antibody has been used with immunofluorescent methods to identify precursors of syncytiotrophoblasts emerging from PSC differentiation. The trophoblasts generated from human PSC most likely represents the primitive placenta encountered in early pregnancy soon after the human trophoblasts invades into the uterine wall.