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Human CRTH-2 Antibody

Catalog #: MAB3338
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB3338
MAB3338-SP
Product Details
Citations (2)
FAQs
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CRTH-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CRTH‑2. Stains human CRTH-2-transfected cells but not parental cells.
Source
Monoclonal Mouse IgG2A Clone # 301108
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human CRTH-2
Met1-Ser395
Accession # Q9Y5Y4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
Human CD3+ peripheral blood lymphocytes
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CRTH-2

CRTH-2, also known as GPR44, is a 7TM G protein-coupled receptor that is expressed by activated Th2 cells and eosinophils. CRTH-2 binds Prostaglandin D2 and induces chemotaxis of these cells in allergic and inflammatory immune responses. Human and mouse CRTH-2 share 78% amino acid sequence identity.

Long Name
Chemoattractant Receptor-homologous Molecule Expressed on Th2 Cells
Entrez Gene IDs
11251 (Human); 14764 (Mouse); 309212 (Rat)
Alternate Names
CD294 antigen; CD294; Chemoattractant receptor-homologous molecule expressed on Th2 cells; CRTH2; CRTH-2; CRTH2chemoattractant receptor homologous molecule expressed on T helper type 2 cells; DL1R; DP2; G protein-coupled receptor 44; GPR44; PGD2R; PTGDR2; putative G-protein coupled receptor 44

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Citations for Human CRTH-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Novel association between vasoactive intestinal peptide and CRTH2 receptor in recruiting eosinophils: a possible biochemical mechanism for allergic eosinophilic inflammation of the airways.
    Authors: El-Shazly A, Begon D, Kustermans G, Arafa M, Dortu E, Henket M, Lefebvre P, Louis R, Delvenne P
    J Biol Chem, 2012-11-20;288(2):1374-84.
    Species: Human
    Sample Types: Whole Cells
    Applications: Blocking
  2. Mast cell-derived prostaglandin D2 controls hyaluronan synthesis in human orbital fibroblasts via DP1 activation: implications for thyroid eye disease.
    Authors: Guo N, Baglole CJ, O'Loughlin CW, Feldon SE, Phipps RP
    J. Biol. Chem., 2010-03-22;285(21):15794-804.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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