Human DGK-zeta Antibody

Catalog #: AF6099 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6099
AF6099-SP

Detection of Human DGK‑ zeta by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DGK-zeta Antibody Summary

Species Reactivity

Human

Specificity

Detects human DGK‑ zeta in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human DGK‑ zeta
Gln800-Val945
Accession # Q13574

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human DGK‑ zeta by Western Blot. Western blot shows lysates of Ramos human Burkitt's lymphoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human DGK-zeta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6099) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for DGK-zeta at approximately 124 & 114 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DGK-zeta

DGK-zeta (Diacylglycerol kinase zeta; also DAG kinase 6) is a 130 kDa member of the eukaryotic diacylglycerol (DAG) kinase family of proteins. It exists in both cytoplasm and nucleus, and is widely expressed, being found in neurons, skeletal muscle cells and endothelium. DGK-zeta converts DAG into phosphatidic acid (PA). In the nucleus, this activates mTOR and promotes cell proliferation. In the cytoplasm, DGK-zeta -generated PA activates PAK1, leading to increased cell motility. Human DGK-zeta is 1117 amino acids (aa) in length and contains two DAG-type Zn-finger regions (aa 287‑419), a MARCKS homology domain (aa 448‑462) and a catalytic domain (aa 486‑607). In addition to the 130 kDa protein, there are several splice variants that run about 114 kDa in SDS-PAGE.

Long Name

Diacylglycerol Kinase

Entrez Gene IDs

8525 (Human); 104418 (Mouse); 81821 (Rat)

Alternate Names

DAGK5; DAGK6DAG kinase zeta; DGKZ; DGKzeta; DGK-zeta; diacylglycerol kinase zeta; diacylglycerol kinase, zeta 104kDa; diacylglycerol kinase, zeta; Diglyceride kinase zeta; EC 2.7.1.107; hDGKzeta