Human DNA-PKcs Antibody

Catalog #: AF3415 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3415
AF3415-SP

Detection of Human DNA-PKcs by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DNA-PKcs Antibody Summary

Species Reactivity

Human

Specificity

Detects human DNA-PK_CSin Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human DNA-PK_CS
Thr3844-Trp4127
Accession # P78527

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human DNA-PKcs by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human DNA-PKcs Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3415) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for DNA-PKcs at approximately 460 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DNA-PKcs

DNA-dependent protein kinase catalytic subunit (DNA-PK_CS) is a member of the PI3-kinase related kinase (PIKK) superfamily. DNA-PK_CS is a serine/threonine kinase that, along with the DNA-binding proteins Ku70/Ku80, binds to regions of DNA double-strand breaks and is involved in double-strand break repair and VDJ recombination.

Long Name

DNA-dependent Protein Kinase Catalytic Subunit

Entrez Gene IDs

5591 (Human); 19090 (Mouse); 360748 (Rat)

Alternate Names

DNA-dependent protein kinase catalytic subunit-like; DNA-dependent protein kinase; DNA-PK catalytic subunit; DNAPK; DNAPKcs; DNA-PKcs; DNPK1; EC 2.7.11.1; HYRC; HYRC1; p350; p460; PRKDC; XRCC7