Human DNA-PKcs Antibody

Catalog #: AF3415 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3415
AF3415-SP

Detection of Human DNA-PKcs by Western Blot.

1 Image

All DNA-PKcs Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DNA-PKcs Antibody Summary

Species Reactivity

Human

Specificity

Detects human DNA-PK_CSin Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human DNA-PK_CS
Thr3844-Trp4127
Accession # P78527

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Customers also Viewed

Detection of Human WASF3/WAVE3 antibody by Western Blot.

Human/Mouse WASF3/WAVE3 Antibody

Cat # AF5515

Citations (2)

4-Hydroxynonenal Antibody

Cat # MAB3249

(3)

Citations (111)

Recombinant Human Sulfamidase/SGSH Protein, CF

Cat # 8380-SU

Citations (1)

CD81 antibody in Jurkat human acute T cell leukemia cell line by Immunocytochemistry (ICC).

Human CD81 MAb (Clone 454729)

Cat # MAB46151

(2)

Mouse B7-H4 Alexa Fluor® 350-conjugated Antibody

Cat # FAB2154U

Mouse Marapsin/Pancreasin Antibody

Cat # AF1989

Human TSLPR Alexa Fluor® 594-conjugated Antibody

Cat # FAB9811T

Recombinant Human CD155/PVR His-tag Alexa Fluor® 647 Protein

Cat # AFR2530

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human DNA-PKcs by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human DNA-PKcs Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3415) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for DNA-PKcs at approximately 460 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DNA-PKcs

DNA-dependent protein kinase catalytic subunit (DNA-PK_CS) is a member of the PI3-kinase related kinase (PIKK) superfamily. DNA-PK_CS is a serine/threonine kinase that, along with the DNA-binding proteins Ku70/Ku80, binds to regions of DNA double-strand breaks and is involved in double-strand break repair and VDJ recombination.

Long Name

DNA-dependent Protein Kinase Catalytic Subunit

Entrez Gene IDs

5591 (Human); 19090 (Mouse); 360748 (Rat)

Alternate Names

DNA-dependent protein kinase catalytic subunit-like; DNA-dependent protein kinase; DNA-PK catalytic subunit; DNAPK; DNAPKcs; DNA-PKcs; DNPK1; EC 2.7.11.1; HYRC; HYRC1; p350; p460; PRKDC; XRCC7