Human DSCAM Long Isoform Antibody Summary
Glu18-Met1595
Accession # O60469
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Recombinant Human DSCAM by Western Blot. Western blot shows 25 ng of Recombinant Human DSCAM (Catalog # 3666-DS) and Recombinant Human DSCAM-L1 (Catalog # 3315-DL). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human DSCAM Long Isoform Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3666) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for DSCAM at approximately 250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
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DSCAM in A172 Human Cell Line. DSCAM was detected in immersion fixed A172 human glioblastoma cell line using 10 µg/mL Goat Anti-Human DSCAM Long Isoform Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3666) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Detection of Mouse Human DSCAM Long Isoform Antibody by Western Blot The C-terminus of DSCAM is not required for protein stability or localization. (C) Western blots of DSCAM protein immunoprecipitated from neonatal brains showed no change in the size or amount of DSCAM in Dscam∆C/∆C mutants. The antibody specificity is confirmed by the lack of signal from Dscam-/- brains. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27637097), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DSCAM
Human DSCAM is a member of the Ig superfamily that maps to a region in chromosome 21 associated with Down Syndrome mental retardation. It encodes a 230 kDa type I membrane protein with a large extracellular domain comprising ten C2 type Ig like domains and six fibronectin type III repeats. The intracellular domain of human DSCAM does not share significant sequence homology with Drosophila DSCAM. Human DSCAM is expressed in axonal and dendritic processes of the central and peripheral nervous system. It is involved in hemophilia interactions and has been implicated in neurogenesis. By alternative splicing, a soluble form of human DSCAM that is identical to the transmembrane form through residue 1571 has been described. The extracellular domain of human and mouse DSCAM share 98% amino acid sequence homology.
Product Datasheets
Citations for Human DSCAM Long Isoform Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
6
Citations: Showing 1 - 6
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DSCAM gene triplication causes excessive GABAergic synapses in the neocortex in Down syndrome mouse models
Authors: H Liu, RN Caballero-, T Hergenrede, T Yang, JM Hull, G Pan, R Li, MW Veling, LL Isom, KY Kwan, ZJ Huang, PG Fuerst, PM Jenkins, B Ye
PloS Biology, 2023-04-20;21(4):e3002078.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
DSCAM regulates delamination of neurons in the developing midbrain
Authors: N Arimura, M Okada, S Taya, KI Dewa, A Tsuzuki, H Uetake, S Miyashita, K Hashizume, K Shimaoka, S Egusa, T Nishioka, Y Yanagawa, K Yamakawa, YU Inoue, T Inoue, K Kaibuchi, M Hoshino
Science Advances, 2020-09-02;6(36):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
DSCAM promotes axon fasciculation and growth in the developing optic pathway
Authors: FM Bruce, S Brown, JN Smith, PG Fuerst, L Erskine
Proc. Natl. Acad. Sci. U.S.A, 2017-01-30;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Replacing the PDZ-interacting C-termini of DSCAM and DSCAML1 with epitope tags causes different phenotypic severity in different cell populations
Elife, 2016-09-16;5(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Neurite arborization and mosaic spacing in the mouse retina require DSCAM.
Authors: Fuerst PG, Koizumi A, Masland RH, Burgess RW
Nature, 2008-01-24;451(7177):470-4.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-Fr, Western Blot -
DSCAM localization and function at the mouse cone synapse.
Authors: de Andrade GB, Long SS, Fleming H et al.
J Comp. Neurol.
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