Human E-Cadherin Antibody

Detection of Human E‑Cadherin by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human E-Cadherin Monoclonal Antibody (Catalog # MAB1838) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for E-Cadherin at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

E-Cadherin in Human Colon. E-Cadherin was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human E-Cadherin Monoclonal Antibody (Catalog # MAB1838) at 2 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human E‑Cadherin by Simple Western^TM. Simple Western lane view shows lysates of A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for E‑Cadherin at approximately 166 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human E‑Cadherin Monoclonal Antibody (Catalog # MAB1838). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Human E-Cadherin by Simple Western D492 and D492M cells carry an epithelial and a mesenchymal phenotype, respectively. (A, B) Relative protein levels of phenotype‐specific markers in D492 and D492M cells as measured by simple western immunoassay (A; representative electropherograms, where the x‐axis shows the protein size (kDa), and the y‐axis indicates signal intensity, reflecting the amount of the protein), and the RPPA (B; average ± SD from three technical replicates). (C) Cell growth shown as increase in confluence (y‐axis) during time after seeding (x‐axis) tracked by the Incucyte (average ± SEM, n ≥ 3). (D) D492 and D492M cell colonies formed during 8‐day growth in 3D Matrigel and stained with phalloidin (red) for labeling F‐actin; scale bar, 50 µm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33759347), licensed under a CC-BY license. Not internally tested by R&D Systems.