Human FHL1 Antibody

Catalog #: AF5938 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5938
AF5938-SP

Detection of Human FHL1 by Western Blot.

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Human FHL1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human FHL1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) FHL2 and rhFHL3 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human FHL1
Thr93-Asn166
Accession # Q13642

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human FHL1 by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human FHL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5938) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for FHL1 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FHL1

FHL1 (Four-and-a-half LIM domains protein 1; also SLIM1 and KyoT1) is a 36 kDa member of the four-and-a half class of the LIM domain-only protein family. It is highly expressed in myocardium and skeletal muscle, and is now known to bind NFATc1, promoting muscle hypertrophy. It is also noted to be a RIP140 binding partner that inhibits estrogen receptor signaling. Human FHL1 is 323 amino acids (aa) in length. It contains one C4-type Zn-finger region (aa 7-31), three distinct LIM domains (aa 40-77; 101-158; 162-212) and a bipartite NLS (aa 231-246). There is one 32 kDa splice variant that shows a 50 aa substitution for aa 231-323. This creates a fourth LIM domain. There are also alternative start sites 29 aa and 16 aa upstream of the standard site, a deletion of aa 168-296, and an 11 aa substitution for aa 81-91. Over aa 93-166, human FHL1 is 97% aa identical to mouse FHL1.

Long Name

Four and a Half LIM Domains Protein 1

Entrez Gene IDs

2273 (Human); 14199 (Mouse); 25177 (Rat)

Alternate Names

bA535K18.1; FHL1; four and a half LIM domains 1; HLH1; HPLH1; KYOT; KYO-T; LIM protein SLIMMER; skeletal muscle LIM-protein1; SLIM1; SLIM1Isl-1 and Mec-3 domains 1; SLIMMER; XMPMA