Human Fibrinogen A Antibody

Catalog #: MAB9898 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9898-100
MAB9898-SP

Detection of Human Fibrinogen A by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Fibrinogen A Antibody Summary

Species Reactivity

Human

Specificity

Detects human Fibrinogen A in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 949330

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Synthetic peptide containing human Fibrinogen A Peptide
Accession # P02671

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Fibrinogen A by Western Blot. Western blot shows human serum (negative control) and human plasma. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Fibrinogen A Monoclonal Antibody (Catalog # MAB9898) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Fibrinogen A at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Fibrinogen A in Human Liver. Fibrinogen A was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Fibrinogen A Monoclonal Antibody (Catalog # MAB9898) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fibrinogen A

Fibrinogen is a 340 kDa, secreted glycoprotein complex that is found in blood at concentrations of 150-400 mg/dL. It is secreted primarily by hepatocytes, but is also reported to be expressed by fibroblasts, type I alveolar epithelium, intestinal epithelium and some tumor cells. Fibrinogen is a homodimer that is composed of two, three-polypeptide chain subunits. In human, each subunit contains one 847amino acid (aa) alpha chain, one 461 aa beta chain, and one 427 aa gamma chain. Multiple interchain disulfide bonds link all three polypeptides. Fibrinogen plays a central role in clot formation. Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers. Fibrinogen is also a component of the ECM and binds to cell surface molecules on inflammatory cells. Mature human alpha, beta and gamma -chains share 67%, 85% and 83% aa identity with mouse alpha, beta and gamma -chains, respectively.

Entrez Gene IDs

2243 (Human); 14161 (Mouse); 361969 (Rat)

Alternate Names

FGA; Fib2; Fibrinogen A; Fibrinogen alpha chain; Fibrinogen alpha subunit; Fibrinogen Type I; fibrinogen, A alpha polypeptide; FPA; MGC119422; MGC119423; MGC119425