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Human Ficolin‑3 Antibody

Catalog #: AF2367
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2367
AF2367-SP
Product Details
Citations (2)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Ficolin‑3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Ficolin-3 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) Ficolin-1 is observed and less 1% cross-reactivity with rhFicolin-2 and rhTenascinR is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Ficolin-3
Lys22-Arg299
Accession # O75636-1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Ficolin‑3 (Catalog # 2367-FC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ficolin-3

Human Ficolin-3 (fibrinogen/collagen-like), also called H-ficolin and, previously, Hakata antigen or thermolabile beta -2 macroglycoprotein, is a member of the ficolin family of secreted pattern recognition proteins that belong to the lectin complement activation pathway (1, 2). Ficolin-3 is expressed by bile duct epithelial cells and hepatocytes, and is released into the bile and circulation, where it averages 18 μg/mL (3, 4). It is also secreted by bronchial and alveolar epithelial cells in the lung (3). Mature human Ficolin-3 shares 46% and 52% amino acid (aa) identity with human Ficolin-1 and Ficolin-2, respectively. Ficolin-3 has only been identified in primates and is likely a pseudogene in other species (5). The 35 kDa, 288 aa human Ficolin-3 (isoform 2) contains a signal sequence, an N-terminal collagen domain and a C‑terminal fibrinogen-like domain that includes a calcium binding site and two potential N-glycosylation sites. Isoform 1 contains an additional 11 aa between the collagen and fibrinogen-like domains. The collagen domain mediates trimer formation, and a ~650 kDa, 18 subunit oligomer is formed by disulfide links at the N‑terminus (2, 6, 7). Ficolin-3 binds a limited set of carbohydrates containing mannose, galactose or D-fucose (2, 6). Binding of microbial carbohydrates has been clearly demonstrated only for the PSA antigen of Aerococcus viridans (4, 8, 9). Pathogen recognition initiates an immune response involving the calcium-dependent interaction of Ficolin-3 with the MBL-associated serine protease (MASP) complex. This cleaves C4 to activate the complement pathway (4, 9). In a secondary role, Ficolins 2 and 3 bind apoptotic cells, activating complement cascades that assist in clearance of the cells (10). Circulating antibodies to Ficolin-3 have been identified in systemic lupus erythematosus (7).

References
  1. Endo, Y. et al. (2006) Adv. Exp. Med. Biol. 586:265.
  2. Sugimoto, R. et al. (1998) J. Biol. Chem. 273:20721.
  3. Akaiwa, M. et al. (1999) J. Histochem. Cytochem. 47:777.
  4. Krarup, A. et al. (2005) Inf. Immun. 72:1052.
  5. Endo, Y. et al. (2004) Genomics 84:737.
  6. Garlatti, V. et al. (2007) EMBO J. 26:623.
  7. Yae, Y. et al. (1991) Biochim. Biophys. Acta 1078:369.
  8. Tsujimura, M. et al. (2001) Clin. Diag. Lab. Immunol. 8:454.
  9. Matsushita, M. et al. (2002) J. Immunol. 168:3502.
  10. Kuraya, M. et al. (2005) Immunobiology 209:689.
Long Name
Ficolin [Collagen/Fibrinogen Domain Containing] 3
Entrez Gene IDs
8547 (Human)
Alternate Names
FCN3; FCNH; Ficolin3; Ficolin-3; HAKA1; H-Ficolin

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Citations for Human Ficolin‑3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. FCN3 functions as a tumor suppressor of lung adenocarcinoma through induction of endoplasmic reticulum stress
    Authors: H Jang, Y Jun, S Kim, E Kim, Y Jung, BJ Park, J Lee, J Kim, S Lee, J Kim
    Cell Death & Disease, 2021-04-15;12(4):407.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Co-Complexes of MASP-1 and MASP-2 Associated with the Soluble Pattern-Recognition Molecules Drive Lectin Pathway Activation in a Manner Inhibitable by MAp44
    Authors: Søren E. Degn, Lisbeth Jensen, Tomasz Olszowski, Jens C. Jensenius, Steffen Thiel
    The Journal of Immunology

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