Human FoxJ1 Antibody Summary
Lys306-Leu421
Accession # Q92949
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human FoxJ1 by Western Blot. Western blot shows lysates of Capan-1 human pancreatic adenocarcinoma cell line and T47D human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human FoxJ1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3619) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FoxJ1 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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FoxJ1 in HEK293 Human Cell Line. FoxJ1 was detected in immersion fixed HEK293 human embryonic kidney cell line using Goat Anti-Human FoxJ1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3619) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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FoxJ1 in Early Proximal Lung Progenitors. FoxJ1 was detected in immersion fixed BGO1V human embryonic stem cells differentiated to early proximal lung progenitors using Goat Anti-Human FoxJ1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3619) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
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Detection of Human FoxJ1 by Simple WesternTM. Simple Western lane view shows lysates of Capan-1 human pancreatic adenocarcinoma cell line and T47D human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for FoxJ1 at approximately 58 kDa (as indicated) using 10 µg/mL of Goat Anti-Human FoxJ1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3619) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FoxJ1
FoxJ1 (Forkhead box protein J1; also HFH4) is a 55-65 kDa, class 1 member of the HNF-3/forkhead gene family of transcription factors. Its observed MW is higher than its predicted MW (45 kDa), suggesting anomalous migration on SDS-PAGE. FoxJ1 promotes the expression of cilia in respiratory, oviduct and choroid plexus epithelium, and maintains T cell tolerance to self-antigens. Human FoxJ1 is 421 amino acids (aa) in length, and contains one forkhead DNA binding domain (aa's 120‑210). The presence of basic residues in the forkhead domain makes FoxJ1 a class 1 Fox protein. There is potential splice variant that contains a 25 aa substation for aa 262-287. Over aa 306-421, human FoxJ1 shares 88% aa with mouse FoxJ1.
Product Datasheets
Citations for Human FoxJ1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Evolution of nasal and olfactory infection characteristics of SARS-CoV-2 variants
Authors: Chen M, Pekosz A, Villano JS et al.
bioRxiv : the preprint server for biology
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Glial progenitor heterogeneity and key regulators revealed by single-cell RNA sequencing provide insight to regeneration in spinal cord injury
Authors: Wei, H;Wu, X;Withrow, J;Cuevas-Diaz Duran, R;Singh, S;Chaboub, LS;Rakshit, J;Mejia, J;Rolfe, A;Herrera, JJ;Horner, PJ;Wu, JQ;
Cell reports
Species: Transgenic Mouse
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
SARS-CoV-2 infection triggers paracrine senescence and leads to a sustained senescence-associated inflammatory response
Authors: Shunya Tsuji, Shohei Minami, Rina Hashimoto, Yusuke Konishi, Tatsuya Suzuki, Tamae Kondo et al.
Nature Aging
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Pulmonary fibrosis distal airway epithelia are dynamically and structurally dysfunctional
Authors: IT Stancil, JE Michalski, D Davis-Hall, HW Chu, JA Park, CM Magin, IV Yang, BJ Smith, E Dobrinskik, DA Schwartz
Nature Communications, 2021-07-27;12(1):4566.
Species: Human
Sample Types: Whole Cells
Applications: IHC -
SARS-CoV-2 infection induces the dedifferentiation of multiciliated cells and impairs mucociliary clearance
Authors: R Robinot, M Hubert, GD de Melo, F Lazarini, T Bruel, N Smith, S Levallois, F Larrous, J Fernandes, S Gellenonco, S Rigaud, O Gorgette, C Thouvenot, C Trébeau, A Mallet, G Duménil, S Gobaa, R Etournay, PM Lledo, M Lecuit, H Bourhy, D Duffy, V Michel, O Schwartz, LA Chakrabart
Nature Communications, 2021-07-16;12(1):4354.
Species: Human
Sample Types: Whole Tissue
Applications: ICC -
BASP1 labels neural stem cells in the neurogenic niches of mammalian brain
Authors: LN Manganas, I Durá, S Osenberg, F Semerci, M Tosun, R Mishra, L Parkitny, JM Encinas, M Maletic-Sa
Scientific Reports, 2021-03-10;11(1):5546.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Osteopontin Expression in Small Airway Epithelium in Copd is Dependent on Differentiation and Confined to Subsets of Cells
Authors: MN Ali, M Mori, TCJ Mertens, P Siddhuraj, JS Erjefält, P Önnerfjord, PS Hiemstra, A Egesten
Sci Rep, 2019-10-29;9(1):15566.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Gefitinib, an EGFR Tyrosine Kinase inhibitor, Prevents Smoke-Mediated Ciliated Airway Epithelial Cell Loss and Promotes Their Recovery
PLoS ONE, 2016-08-17;11(8):e0160216.
Species: Human
Sample Types: Whole Cells
Applications: IHC-P -
Hyaluronan fragments/CD44 mediate oxidative stress-induced MUC5B up-regulation in airway epithelium.
Authors: Casalino-Matsuda SM, Monzon ME, Day AJ, Forteza RM
Am. J. Respir. Cell Mol. Biol., 2008-08-28;40(3):277-85.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Submersion and Hypoxia Inhibit Ciliated Cell Differentiation in a Notch Dependent Manner..
Authors: Gerovac BJ, Valencia M, Baumlin N et al.
Am. J. Respir. Cell Mol. Biol.
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