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Human Galectin-1 Antibody

Catalog #: AF1152
22 Citations 1 Review Datasheet / COA / SDS
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AF1152
AF1152-SP
Detection of Human Galectin‑1 by Western Blot.
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Citations (22)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Galectin-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Galectin-1 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Simple Western
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Galectin-1 antibody by Western Blot. View Larger

Detection of Human Galectin‑1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and A549 human lung carcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Galectin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1152) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for Galectin-1 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Galectin-1 antibody in Human Prostate Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

Galectin‑1 in Human Prostate Cancer Tissue. Galectin-1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 1.7 µg/mL Goat Anti-Human Galectin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1152) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry Galectin-1 antibody in Human Prostate Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

Galectin‑1 in Human Prostate Cancer Tissue. Galectin-1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human Galectin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1152) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of stromal cells and nuclei of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Intracellular Staining by Flow Cytometry Detection of Galectin-1 antibody in Human Blood Monocytes antibody by Flow Cytometry. View Larger

Detection of Galectin‑1 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (FAB3832P) and either (A) Normal Goat IgG Control (AB-108-C) or (B) Goat Anti-Human Galectin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1152) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Simple Western Detection of Human Galectin-1 antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human Galectin‑1 by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin-1 at approximately 17 kDa (as indicated) using 1 µg/mL of Goat Anti-Human Galectin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1152) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Galectin-1

The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified to date. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins.

Galectin-1, also known as L-14, BHL and galaptin, is a monomeric or homodimeric prototype galectin that is expressed in a variety of cells and tissues including muscle, heart, liver, prostate, lymph nodes, spleen, thymus, placenta, testis, retina, macrophages, B cells, T cells, dendritic cells, and tumor cells. It preferentially binds laminin, fibronectin, 90K/Mac-2BP, CD45, CD43, CD7, CD2, CD3, and ganglioside GM1. Galectin-1 modulates cell growth and proliferation, either positively or negatively, depending on the cell type and activation status. It controls cell survival by inducing apoptosis of activated T cells and immature thymocytes. It modulates cytokine secretion by inducing Th2 type cytokines and inhibiting pro-inflammatory cytokine production. Galectin-1 can also modulate cell-cell as well as cell-matrix interactions and depending on the cell type and developmental stage, promote cell attachment or detachment. Galectin-1 has immunosuppressive and anti-inflammatory properties and has been shown to suppress acute and chronic inflammation and autoimmunity. Human and mouse galectin-1 share about 88% amino acid sequence similarity.

Entrez Gene IDs
3956 (Human); 16852 (Mouse)
Alternate Names
14 kDa laminin-binding protein; 14 kDa lectin; Beta-galactoside-binding lectin L-14-I; BHL; DKFZp686E23103; GAL1; gal-1; Galaptin; galectin 1; Galectin1; Galectin-1; GBP; HBL; HLBP14; HPL; L-14; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; lectin, galactoside-binding, soluble, 1; LGALS1; Putative MAPK-activating protein PM12; S-Lac lectin 1

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Citations for Human Galectin-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. Distinct features of the host-parasite interactions between nonadherent and adherent Trichomonas vaginalis isolates
    Authors: HM Hsu, YY Yang, YH Huang, CH Chu, TJ Tu, YT Wu, CJ Chiang, SB Yang, DK Hsu, FT Liu, JH Tai
    PloS Neglected Tropical Diseases, 2023-01-03;17(1):e0011016.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  2. A human breast atlas integrating single-cell proteomics and transcriptomics
    Authors: G. Kenneth Gray, Carman Man-Chung Li, Jennifer M. Rosenbluth, Laura M. Selfors, Nomeda Girnius, Jia-Ren Lin et al.
    Developmental Cell
  3. Galectin-1 prevents pathological vascular remodeling in atherosclerosis and abdominal aortic aneurysm
    Authors: R Roldán-Mon, JM Pérez-Sáez, I Cerro-Pard, J Oller, D Martinez-L, E Nuñez, SM Maller, C Gutierrez-, N Mendez-Bar, JC Escola-Gil, JB Michel, M Mittelbrun, J Vázquez, LM Blanco-Col, GA Rabinovich, JL Martin-Ven
    Science Advances, 2022-03-16;8(11):eabm7322.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Multi-Faceted Effects of ST6Gal1 Expression on Precursor B-Lineage Acute Lymphoblastic Leukemia
    Authors: Mingfeng Zhang, Tong Qi, Lu Yang, Daniel Kolarich, Nora Heisterkamp
    Frontiers in Oncology
  5. A photo-cross-linking GlcNAc analog enables covalent capture of N-linked glycoprotein-binding partners on the cell surface
    Authors: Han Wu, Asif Shajahan, Jeong-Yeh Yang, Emanuela Capota, Amberlyn M. Wands, Connie M. Arthur et al.
    Cell Chemical Biology
  6. Integration of T helper and BCR signals governs enhanced plasma cell differentiation of memory B cells by regulation of CD45 phosphatase activity
    Authors: P Szodoray, TK Andersen, J Heinzelbec, JF Imbery, PC Huszthy, SM Stanford, B Bogen, OB Landsverk, N Bottini, A Tveita, LA Munthe, B Nakken
    Cell Reports, 2021-08-10;36(6):109525.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Co-Immunoprecipitation, ICC
  7. Schwann Cell Stimulation of Pancreatic Cancer Cells: A Proteomic Analysis
    Authors: Aysha Ferdoushi, Xiang Li, Nathan Griffin, Sam Faulkner, M. Fairuz B. Jamaluddin, Fangfang Gao et al.
    Frontiers in Oncology
  8. Galectin-1–driven T cell exclusion in the tumor endothelium promotes immunotherapy resistance
    Authors: Dhanya K. Nambiar, Todd Aguilera, Hongbin Cao, Shirley Kwok, Christina Kong, Joshua Bloomstein et al.
    Journal of Clinical Investigation
  9. Glucocorticoid receptor inhibits M�ller glial galectin-1 expression via DUSP1-dependent and -independent deactivation of AP-1 signalling
    Authors: I Hirose, A Kanda, K Noda, S Ishida
    J. Cell. Mol. Med., 2019-07-21;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Treatment of B-cell precursor acute lymphoblastic leukemia with the Galectin-1 inhibitor PTX008
    Authors: H Paz, EJ Joo, CH Chou, F Fei, KH Mayo, H Abdel-Azim, H Ghazarian, J Groffen, N Heisterkam
    J. Exp. Clin. Cancer Res., 2018-03-27;37(1):67.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Galectins-1, -3, and -7 Are Prognostic Markers for Survival of Ovarian Cancer Patients
    Authors: H Schulz, E Schmoeckel, C Kuhn, S Hofmann, D Mayr, S Mahner, U Jeschke
    Int J Mol Sci, 2017-06-08;18(6):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  12. Induction of galectin-1 by TLR-dependent PI3K activation enhances epithelial-mesenchymal transition of metastatic ovarian cancer cells
    Authors: Ga Bin Park, Yoon Hee Chung, Daejin Kim
    Oncology Reports
  13. Serum galectin-1 in patients with multiple myeloma: associations with survival, angiogenesis, and biomarkers of macrophage activation
    Authors: MN Andersen, M Ludvigsen, N Abildgaard, I Petruskevi, R Hjortebjer, M Bjerre, B Honoré, HJ Møller, NF Andersen
    Onco Targets Ther, 2017-04-04;10(0):1977-1982.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development (Capture)
  14. Prototype and Chimera-Type Galectins in Placentas with Spontaneous and Recurrent Miscarriages
    Int J Mol Sci, 2016-04-28;17(5):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  15. Expression of immune checkpoint molecules in endometrial carcinoma
    Authors: JIA LIU, YULING LIU, WULIANG WANG, CHENYANG WANG, YANHONG CHE
    Experimental and Therapeutic Medicine
  16. The luteotrophic function of galectin-1 by binding to the glycans on vascular endothelial growth factor receptor-2 in bovine luteal cells
    Authors: Masahiro SANO, Kazuhisa HASHIBA, Junko NIO-KOBAYASHI, Kiyoshi OKUDA
    Journal of Reproduction and Development
  17. Graves' disease is associated with a defective expression of the immune regulatory molecule galectin-9 in antigen-presenting dendritic cells.
    Authors: Leskela S, Serrano A, de la Fuente H, Rodriguez-Munoz A, Ramos-Levi A, Sampedro-Nunez M, Sanchez-Madrid F, Gonzalez-Amaro R, Marazuela M
    PLoS ONE, 2015-04-16;10(4):e0123938.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC-Fr
  18. Melanoma Cell Galectin-1 Ligands Functionally Correlate with Malignant Potential.
    Authors: Yazawa E, Geddes-Sweeney J, Cedeno-Laurent F, Walley K, Barthel S, Opperman M, Liang J, Lin J, Schatton T, Laga A, Mihm M, Qureshi A, Widlund H, Murphy G, Dimitroff C
    J Invest Dermatol, 2015-03-10;135(7):1849-62.
    Species: Human, Mouse
    Sample Types: Tissue Homogenates, Whole Cells
    Applications: Flow Cytometry, Western Blot
  19. Tonsil-derived mesenchymal stem cells alleviate concanavalin A-induced acute liver injury.
    Authors: Ryu K, Kim S, Kim Y, Woo S, Sung S, Kim H, Jung S, Jo I, Park J
    Exp Cell Res, 2014-06-19;326(1):143-54.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  20. Galectin-1 promotes lung cancer progression and chemoresistance by upregulating p38 MAPK, ERK, and cyclooxygenase-2.
    Authors: Chung L, Tang S, Sun G, Chou T, Yeh T, Yu S, Sun K
    Clin Cancer Res, 2012-06-13;18(15):4037-47.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  21. Galectin-1-mediated cell death is increased by CD30-induced signaling in anaplastic large cell lymphoma cells but not in Hodgkin lymphoma cells.
    Authors: Suzuki O, Hirsch B, Abe M, Durkop H, Stein H
    Lab. Invest., 2011-10-10;92(2):191-9.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  22. Sialylation of beta1 integrins blocks cell adhesion to galectin-3 and protects cells against galectin-3-induced apoptosis.
    Authors: Zhuo Y, Chammas R, Bellis SL
    J. Biol. Chem., 2008-08-08;283(32):22177-85.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Human Galectin-1 Antibody
By Juliana Redondo on 11/24/2016
Application: WB Sample Tested: Mesenchymal stem cells Species: Human
Western Blot Human Galectin-1 Antibody AF1152