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Human Gas6 Antibody

Catalog #: AB885
13 Citations 2 Reviews Datasheet / COA / SDS
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AB885
Product Details
Citations (13)
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Supplemental Products
Reviews (2)
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Human Gas6 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Gas6 in ELISAs and Western blots. Because this antibody preparation is a total IgG fraction, complete monospecificity cannot be assumed.
Source
Polyclonal Goat IgG
Purification
Protein A or G purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Gas6
Asp118-Ala678
Accession # NP_000811
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Gas6 (Catalog # 885-GS)
Blockade of Receptor-ligand Interaction
In a functional ELISA, 1-5 µg/mL of this antibody will block 50% of the binding of 10 ng/mL of biotinylated Recombinant Human Gas6, lacking the Gla domain, to immobilized Recombinant Human Axl Fc Chimera (Catalog # 154-AL) coated at 2 µg/mL (100 µL/well). At 100 μg/mL, this antibody will block >90% of the binding.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 1 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Gas6

Gas6 (Growth Arrest Specific 6) is a multimodular protein that is upregulated by a wide variety of cell types in response to growth arrest (1). Gas6 and the structurally related Protein S are vitamin K-dependent and have an extensively gamma -carboxylated N-terminal Gla domain, four EGF‑like repeats, and a C-terminal region with homology to steroid hormone binding globulin (SHBG) (2). Human Gas6 is a 75 kDa protein that shares 77-79% aa sequence identity with mouse and rat Gas6, and 43% aa identity with human protein S (over the region expressed). Alternate splicing generates isoforms that lack the Gla domain and/or the spacer between the EGF‑like and SHBG regions. Gas6 binds and induces signaling through the receptor tyrosine kinases Axl, Dtk, and Mer (3-5). Human Gas6 interacts with both mouse and rat orthologs of these receptors (1). The full length isoform may be cleaved, resulting in release of the free SHBG region which can independently activate Axl (6). Shed soluble forms of Axl and Mer bind Gas6 and function as decoy receptors (7, 8). Gas6 induces a variety of responses, including prevention of apoptosis (9), cell proliferation (10), platelet-mediated thrombosis (11), retinal epithelial cell phagocytosis of outer rod segments (12), inhibition of VEGF-induced endothelial cell chemotaxis (13), and the differentiation and expansion of NK cell precursors (14). The affinity of Gas6 for phosphatidylserine likely contributes to its role in promoting the phagocytosis of apoptotic cells (15). Several of these effects have been shown to require gamma -carboxylation of the Gla domain (12, 16).

References
  1. Hafizi, S. and B. Dahlback (2006) FEBS J. 273:5231.
  2. Manfioletti, G. et al. (1993) Mol. Cell Biol. 13:4976.
  3. Stitt, T.N. et al. (1995) Cell 80:661.
  4. Ohashi, K. et al. (1995) J. Biol. Chem. 270:22681.
  5. Nagata, K. et al. (1996) J. Biol. Chem. 271:30022.
  6. Goruppi, S. et al. (1997) FEBS Lett. 415:59.
  7. Sather, S. et al. (2007) Blood 109:1026.
  8. Budagian, V. et al. (2005) Mol. Cell. Biol. 25:9324.
  9. Shankar, S.L. et al. (2006) J. Neurosci. 26:5638.
  10. Yanagita, M. et al. (2002) J. Clin. Invest. 110:239.
  11. Gould, W.R. et al. (2005) J. Thromb. Haemost. 3:733.
  12. Hall, M.O. et al. (2002) Exp. Eye Res. 75:391.
  13. Gallicchio, M. et al. (2005) Blood 105:1970.
  14. Caraux, A. et al. (2006) Nat. Immunol. 7:747.
  15. Wu, Y. et al. (2005) J. Cell Sci. 118:539.
  16. Hasanbasic, I. et al. (2005) J. Thromb. Haemost. 3:2790.
Long Name
Growth-arrest-specific Protein 6
Entrez Gene IDs
2621 (Human); 14456 (Mouse)
Alternate Names
AXLLG; AXLLGAXL stimulatory factor; AXSFAXL receptor tyrosine kinase ligand; DKFZp666G247; FLJ34709; Gas6; GAS-6; growth arrest-specific 6; growth arrest-specific protein 6

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Citations for Human Gas6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. AXL and GAS6 co-expression in lung adenocarcinoma as a prognostic classifier
    Authors: M Seike, CH Kim, F Zou, R Noro, M Chiba, A Ishikawa, S ?unugi, K Kubota, A Gemma
    Oncol. Rep., 2017-04-21;0(0):.
  2. CNK2 promotes cancer cell motility by mediating ARF6 activation downstream of AXL signalling
    Authors: Serwe, G;Kachaner, D;Gagnon, J;Plutoni, C;Lajoie, D;Duramé, E;Sahmi, M;Garrido, D;Lefrançois, M;Arseneault, G;Saba-El-Leil, MK;Meloche, S;Emery, G;Therrien, M;
    Nature communications
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Anti-inflammatory clearance of amyloid-beta by a chimeric Gas6 fusion protein
    Authors: H Jung, SY Lee, S Lim, HR Choi, Y Choi, M Kim, S Kim, Y Lee, KH Han, WS Chung, CH Kim
    Nature Medicine, 2022-08-04;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. Zika virus infects pericytes in the choroid plexus and enters the central nervous system through the blood-cerebrospinal fluid barrier
    Authors: J Kim, B Alejandro, M Hetman, EM Hattab, J Joiner, H Schroten, H Ishikawa, DH Chung
    PLoS Pathog., 2020-05-01;16(5):e1008204.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Axl Promotes Zika Virus Entry and Modulates the Antiviral State of Human Sertoli Cells
    Authors: DP Strange, B Jiyarom, N Pourhabibi, X Xie, C Baker, H Sadri-Arde, PY Shi, S Verma
    MBio, 2019-07-16;10(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction.
    Authors: Zizzo G, Hilliard B, Monestier M, Cohen P
    J Immunol, 2012-08-31;189(7):3508-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Activation of the AXL kinase causes resistance to EGFR-targeted therapy in lung cancer.
    Authors: Zhang Z, Lee JC
    Nat. Genet., 2012-07-01;44(8):852-860.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-P, Western Blot
  8. Differential expression of Axl and Gas6 in renal cell carcinoma reflecting tumor advancement and survival.
    Authors: Gustafsson A, Martuszewska D, Johansson M, Ekman C, Hafizi S, Ljungberg B, Dahlback B
    Clin. Cancer Res., 2009-06-30;15(14):4742-9.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  9. Development of a novel immunoassay for the assessment of plasma Gas6 concentrations and their variation with hormonal status.
    Authors: Clauser S, Peyrard S, Gaussem P, Crespin M, Emmerich J, Aiach M, Borgel D
    Clin. Chem., 2007-08-23;53(10):1808-13.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  10. A high Gas6 level in plasma predicts venous thromboembolism recurrence, major bleeding and mortality in the elderly: a prospective multicenter cohort study
    Authors: Annatina Schnegg‐Kaufmann, Sara Calzavarini, Andreas Limacher, Marie Mean, Marc Righini, Daniel Staub et al.
    Journal of Thrombosis and Haemostasis
  11. Blockade of Stromal Gas6 Alters Cancer Cell Plasticity, Activates NK Cells, and Inhibits Pancreatic Cancer Metastasis
    Authors: Lucy Ireland, Teifion Luckett, Michael C. Schmid, Ainhoa Mielgo
    Frontiers in Immunology
  12. Study of Early Elevated Gas6 Plasma Level as a Predictor of Mortality in a Prospective Cohort of Patients with Sepsis
    Authors: Grégoire Stalder, Yok Ai Que, Sara Calzavarini, Laurent Burnier, Christophe Kosinski, Pierluigi Ballabeni et al.
    PLOS ONE
  13. GAS6-expressing and self-sustaining cancer cells in 3D spheroids activate the PDK-RSK-mTOR pathway for survival and drug resistance
    Authors: Christine Baumann, Axel Ullrich, Robert Torka
    Molecular Oncology

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Human Gas6 Antibody
By Anonymous on 06/17/2017
Application: WB Sample Tested: Cell Culture Media Species: Human
Human Gas6 Antibody
By Adam Guess on 01/10/2017
Application: Flow Sample Tested: Mesenchymal stem cells Species: Human
Flow Cytometry Human Gas6 Antibody AB885