Human GATA-1 Antibody

Catalog #: MAB1779
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1779
MAB1779-SP

All GATA-1 Antibodies

Product Details

Citations (2)

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GATA-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human GATA-1 in direct ELISAs. In Western blots, this antibody does not cross-react with rhGATA-2 or rhGATA-6.

Source

Monoclonal Rat IgG_2B Clone # 234732

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human GATA-1
Met1-Ser413
Accession # P15976

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human GATA‑1

Immunocytochemistry

8-25 µg/mL

Immersion-fixed MG-63 human osteosarcoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GATA-1

GATA-1 is the founding member of the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue‑specific programs of gene expression and morphogenesis. GATA-1 is expressed in blood forming cells. It interacts with several additional proteins to activate or repress gene expression and is essential for erythropoiesis (1).

References

Patient, R.K. and J. McGhee (2002) Curr. Opin. Genet. Dev. 12:416.

Entrez Gene IDs

2623 (Human); 14460 (Mouse)

Alternate Names

Eryf1; ERYF1GATA-binding protein 1 (globin transcription factor 1); GATA binding protein 1 (globin transcription factor 1); GATA1; GATA-1; GATA-1erythroid transcription factor; GATA-binding factor 1; GF-1; GF1globin transcription factor 1; NF-E1 DNA-binding protein; NFE1erythroid transcription factor 1; transcription factor GATA1; XLTT

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human GATA-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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