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Human GATA-3 Antibody

Catalog #: AF2605
43 Citations 1 Review Datasheet / COA / SDS
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AF2605
AF2605-SP
Detection of Human and Mouse GATA‑3 by Western Blot.
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Citations (43)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GATA-3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human and mouse GATA-3 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human GATA-3
Pro135-Ser258
Accession # P23771
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Simple Western
2.5 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse GATA-3 antibody by Western Blot. View Larger

Detection of Human and Mouse GATA‑3 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, and EL-4 mouse lymphoblast cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human GATA-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2605) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for GATA-3 full length (FL) at approximately 55 kDa and the splice form (SF) at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry GATA‑3 antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

GATA‑3 in Human Breast Cancer Tissue. GATA-3 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human GATA-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2605) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunocytochemistry GATA‑3 antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC). View Larger

GATA‑3 in MCF-7 Human Cell Line. GATA-3 was detected in immersion fixed MCF-7 human breast cancer cell line using 10 µg/mL Goat Anti-Human GATA-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2605) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry GATA‑3 antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

GATA‑3 in Human Breast Cancer Tissue. GATA-3 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human GATA-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2605) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Human GATA‑3 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human GATA‑3 by Simple WesternTM. Simple Western lane view shows lysates of MCF-7 human breast cancer cell line and Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. Specific bands were detected for GATA-3 full length (FL) at approximately 62 kDa and the splice form (SF) at approximately 52 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Human GATA-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2605) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GATA-3

GATA-3 belongs to the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. It is widely expressed in mesodermal- and endodermal-derived tissues. GATA-3 has been shown to be an essential regulator for immune cell function, sympathetic neuron development and the maintenance of the differentiated state in epithelial cells.

Entrez Gene IDs
2625 (Human); 14462 (Mouse)
Alternate Names
131320; GATA binding protein 3; GATA3; GATA-3; GATA-binding factor 3; GATA-binding protein 3; HDRMGC5199; MGC2346; MGC5445; trans-acting T-cell-specific transcription factor GATA-3

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Citations for Human GATA-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

43 Citations: Showing 1 - 10
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  1. Human embryo polarization requires PLC signaling to mediate trophectoderm specification
    Authors: Meng Zhu, Marta Shahbazi, Angel Martin, Chuanxin Zhang, Berna Sozen, Mate Borsos et al.
    eLife
  2. Generation of kidney organoids from human pluripotent stem cells
    Authors: Minoru Takasato, Pei X Er, Han S Chiu, Melissa H Little
    Nature Protocols
  3. Diffuse intrinsic pontine tumors: a study of primitive neuroectodermal tumors versus the more common diffuse intrinsic pontine gliomas
    Authors: Alexandra Sufit, Andrew M. Donson, Diane K. Birks, Jeffrey A. Knipstein, Laura Z. Fenton, Paul Jedlicka et al.
    Journal of Neurosurgery: Pediatrics
  4. Development of a human iPSC-derived placental barrier-on-chip model
    Authors: Agathe Lermant, Gwenaëlle Rabussier, Henriëtte L. Lanz, Lindsay Davidson, Iain M. Porter, Colin E. Murdoch
    iScience
  5. Single cell analysis of the developing mouse kidney provides deeper insight into marker gene expression and ligand-receptor crosstalk
    Authors: Alexander N. Combes, Belinda Phipson, Kynan T. Lawlor, Aude Dorison, Ralph Patrick, Luke Zappia et al.
    Development
  6. 3D mouse embryonic stem cell culture for generating inner ear organoids.
    Authors: Koehler KR, Hashino E.
    Nat Protoc
  7. Enhanced metanephric specification to functional proximal tubule enables toxicity screening and infectious disease modelling in kidney organoids
    Authors: Jessica M. Vanslambrouck, Sean B. Wilson, Ker Sin Tan, Ella Groenewegen, Rajeev Rudraraju, Jessica Neil et al.
    bioRxiv
  8. Reporter‐based fate mapping in human kidney organoids confirms nephron lineage relationships and reveals synchronous nephron formation
    Authors: Sara E Howden, Jessica M Vanslambrouck, Sean B Wilson, Ker Sin Tan, Melissa H Little
    EMBO reports
  9. Human iPSC-derived renal organoids engineered to report oxidative stress can predict drug-induced toxicity
    Authors: Lawrence M, Elhendawi M, Morlock M et al.
    iScience
  10. Conserved and Divergent Molecular and Anatomic Features of Human and Mouse Nephron Patterning
    Authors: Nils O. Lindström, Tracy Tran, Jinjin Guo, Elisabeth Rutledge, Riana K. Parvez, Matthew E. Thornton et al.
    Journal of the American Society of Nephrology
  11. Influence of glucose and oxygen tension on the trophectoderm and the inner cell mass of in vitro produced bovine embryos
    Authors: Berling, FP;Mendes, CM;Goissis, MD;
    Theriogenology
    Species: Bovine
    Sample Types: Embryo
    Applications: Immunohistochemistry
  12. Fetal Kidney Grafts and Organoids from Microminiature Pigs: Establishing a Protocol for Production and Long-Term Cryopreservation
    Authors: Inage, Y;Fujimori, K;Takasu, M;Matsui, K;Kinoshita, Y;Morimoto, K;Koda, N;Yamamoto, S;Shimada, K;Yokoo, T;Kobayashi, E;
    International journal of molecular sciences
    Species: Porcine, Xenograft
    Sample Types: Whole Cells, Whole Tissue
    Applications: Immunohistochemistry, Immunocytochemistry
  13. Successful reconstruction of the rat ureter by a syngeneic collagen tube with a cardiomyocyte sheet
    Authors: Yamamoto, S;Matsui, K;Kinoshita, Y;Hiroshi Sasaki, ;Sekine, H;Saito, Y;Nakayama, Y;Kume, H;Kimura, T;Yokoo, T;Kobayashi, E;
    Regenerative therapy
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  14. Large-scale production of human blastoids amenable to modeling blastocyst development and maternal-fetal cross talk
    Authors: Yu, L;Logsdon, D;Pinzon-Arteaga, CA;Duan, J;Ezashi, T;Wei, Y;Ribeiro Orsi, AE;Oura, S;Liu, L;Wang, L;Liu, K;Ding, X;Zhan, L;Zhang, J;Nahar, A;Stobbe, C;Katz-Jaffe, M;Schoolcraft, WB;Tan, T;Hon, GC;Yuan, Y;Wu, J;
    Cell stem cell
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  15. Cynomolgus monkey embryo model captures gastrulation and early pregnancy
    Authors: J Li, Q Zhu, J Cao, Y Liu, Y Lu, Y Sun, Q Li, Y Huang, S Shang, X Bian, C Li, L Zhang, Y Wang, Y Nie, J Fu, W Li, MA Mazid, Y Jiang, W Jia, X Wang, Y Sun, MA Esteban, Q Sun, F Zhou, Z Liu
    Cell Stem Cell, 2023-04-06;30(4):362-377.e7.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Types: Embryo
    Applications: Immunohistochemistry
  16. Organoid-on-a-chip model of human ARPKD reveals mechanosensing pathomechanisms for drug discovery
    Authors: K Hiratsuka, T Miyoshi, KT Kroll, NR Gupta, MT Valerius, T Ferrante, M Yamashita, JA Lewis, R Morizane
    Science Advances, 2022-09-21;8(38):eabq0866.
    Species: Human
    Sample Types: Organoids
    Applications: IHC
  17. Delamination of trophoblast-like syncytia from the amniotic ectodermal analogue in human primed embryonic stem cell-based differentiation model
    Authors: M Ohgushi, N Taniyama, A Vandenbon, M Eiraku
    Cell Reports, 2022-06-21;39(12):110973.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  18. Integrated pseudotime analysis of human pre-implantation embryo single-cell transcriptomes reveals the dynamics of lineage specification
    Authors: D Meisterman, A Bruneau, S Loubersac, A Reignier, J Firmin, V François-C, S Kilens, Y Lelièvre, J Lammers, M Feyeux, P Hulin, S Nedellec, B Bretin, G Castel, N Allègre, S Covin, A Bihouée, M Soumillon, T Mikkelsen, P Barrière, C Chazaud, J Chappell, V Pasque, J Bourdon, T Fréour, L David
    Cell Stem Cell, 2021-05-17;0(0):.
    Species: Human
    Sample Types: Embryo
    Applications: IHC
  19. Plasticity of distal nephron epithelia from human kidney organoids enables the induction of ureteric tip and stalk
    Authors: SE Howden, SB Wilson, E Groenewege, L Starks, TA Forbes, KS Tan, JM Vanslambro, EM Holloway, YH Chen, S Jain, JR Spence, MH Little
    Cell Stem Cell, 2020-12-29;0(0):.
    Species: Human
    Sample Types: Organoid
    Applications: Immunohistochemistry
  20. Human Pluripotent Stem Cell-Derived Kidney Organoids with Improved Collecting Duct Maturation and Injury Modeling
    Authors: K Uchimura, H Wu, Y Yoshimura, BD Humphreys
    Cell Reports, 2020-12-15;33(11):108514.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  21. Developmental potential of aneuploid human embryos cultured beyond implantation
    Authors: MN Shahbazi, T Wang, X Tao, BAT Weatherbee, L Sun, Y Zhan, L Keller, GD Smith, A Pellicer, RT Scott, E Seli, M Zernicka-G
    Nat Commun, 2020-08-10;11(1):3987.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  22. Gut Microbiota Modulate CD8�T Cell Responses to Influence Colitis-Associated Tumorigenesis
    Authors: AI Yu, L Zhao, KA Eaton, S Ho, J Chen, S Poe, J Becker, A Gonzalez, D McKinstry, M Hasso, J Mendoza-Ca, J Whitfield, C Koumpouras, PD Schloss, EC Martens, GY Chen
    Cell Rep, 2020-04-07;31(1):107471.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  23. Mouse Embryonic Stem Cell-Derived Ureteric Bud Progenitors Induce Nephrogenesis
    Authors: Z Tan, A Rak-Raszew, I Skovorodki, SJ Vainio
    Cells, 2020-01-31;9(2):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  24. Pyrrothiogatain acts as an inhibitor of GATA family proteins and inhibits Th2 cell differentiation in vitro
    Authors: S Nomura, H Takahashi, J Suzuki, M Kuwahara, M Yamashita, T Sawasaki
    Sci Rep, 2019-11-22;9(1):17335.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP
  25. Tubuloids derived from human adult kidney and urine for personalized disease modeling
    Authors: F Schutgens, MB Rookmaaker, T Margaritis, A Rios, C Ammerlaan, J Jansen, L Gijzen, M Vormann, A Vonk, M Viveen, FY Yengej, S Derakhshan, KM de Winter-, B Artegiani, R van Boxtel, E Cuppen, APA Hendrickx, MM van den He, E Heitzer, H Lanz, J Beekman, JL Murk, R Masereeuw, F Holstege, J Drost, MC Verhaar, H Clevers
    Nat. Biotechnol., 2019-03-04;37(3):303-313.
    Species: Human
    Sample Types: Organoids
    Applications: IHC
  26. Evaluation of variability in human kidney organoids
    Authors: B Phipson, PX Er, AN Combes, TA Forbes, SE Howden, L Zappia, HJ Yen, KT Lawlor, LJ Hale, J Sun, E Wolvetang, M Takasato, A Oshlack, MH Little
    Nat. Methods, 2018-12-20;16(1):79-87.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  27. Trophoblast organoids as a model for maternal-fetal interactions during human placentation
    Authors: MY Turco, L Gardner, RG Kay, RS Hamilton, M Prater, MS Hollinshea, A McWhinnie, L Esposito, R Fernando, H Skelton, F Reimann, FM Gribble, A Sharkey, SGE Marsh, S O'Rahilly, M Hemberger, GJ Burton, A Moffett
    Nature, 2018-11-28;0(0):.
    Species: Human
    Sample Types: Trophoblast Organoids
    Applications: IHC
  28. GATA3 targets semaphorin 3B in mammary epithelial cells to suppress breast cancer progression and metastasis
    Authors: P Shahi, CY Wang, J Chou, C Hagerling, H Gonzalez V, A Ruderisch, Y Yu, MD Lai, Z Werb
    Oncogene, 2017-06-05;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  29. Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis.
    Authors: Takasato M, Er P, Chiu H, Maier B, Baillie G, Ferguson C, Parton R, Wolvetang E, Roost M, Chuva de Sousa Lopes S, Little M
    Nature, 2015-10-07;526(7574):564-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  30. Human cytomegalovirus infection interferes with the maintenance and differentiation of trophoblast progenitor cells of the human placenta.
    Authors: Tabata T, Petitt M, Zydek M, Fang-Hoover J, Larocque N, Tsuge M, Gormley M, Kauvar L, Pereira L
    J Virol, 2015-03-04;89(9):5134-47.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-P
  31. A Gata3-Mafb transcriptional network directs post-synaptic differentiation in synapses specialized for hearing.
    Authors: Yu, Wei-Ming, Appler, Jessica, Kim, Ye-Hyun, Nishitani, Allison, Holt, Jeffrey, Goodrich, Lisa V
    Elife, 2013-12-10;2(0):e01341.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  32. Cytokines inhibit norepinephrine transporter expression by decreasing Hand2.
    Authors: Pellegrino MJ, Parrish DC, Zigmond RE, Habecker BA
    Mol. Cell. Neurosci., 2011-01-15;46(3):671-80.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  33. Critical role for GATA3 in mediating Tie2 expression and function in large vessel endothelial cells.
    Authors: Song H, Suehiro J, Kanki Y, Kawai Y, Inoue K, Daida H, Yano K, Ohhashi T, Oettgen P, Aird WC, Kodama T, Minami T
    J. Biol. Chem., 2009-08-12;284(42):29109-24.
    Species: Human, Primate - Cholrocebus pygerythrus (Vervet Monkey)
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  34. Novel immunomodulatory properties of berbamine through selective down-regulation of STAT4 and action of IFN-gamma in experimental autoimmune encephalomyelitis.
    Authors: Ren Y, Lu L, Guo TB, Qiu J, Yang Y, Liu A, Zhang JZ
    J. Immunol., 2008-07-15;181(2):1491-8.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  35. Gata3 Is a Critical Regulator of Cochlear Wiring
    Authors: Jessica M. Appler, Cindy C. Lu, Noah R. Druckenbrod, Wei-Ming Yu, Edmund J. Koundakjian, Lisa V. Goodrich
    The Journal of Neuroscience
  36. Beneficial Impact of Interspecies Chimeric Renal Organoids Against a Xenogeneic Immune Response
    Authors: Yatsumu Saito, Naoto Matsumoto, Shuichiro Yamanaka, Takashi Yokoo, Eiji Kobayashi
    Frontiers in Immunology
  37. Accelerated protocol for the differentiation of podocytes from human pluripotent stem cells
    Authors: Julie Bejoy, Eddie Spencer Qian, Lauren Elizabeth Woodard
    STAR Protocols
  38. Enhanced metanephric specification to functional proximal tubule enables toxicity screening and infectious disease modelling in kidney organoids
    Authors: Jessica M. Vanslambrouck, Sean B. Wilson, Ker Sin Tan, Ella Groenewegen, Rajeev Rudraraju, Jessica Neil et al.
    Nature Communications
  39. Comprehensive transcriptome analysis of cochlear spiral ganglion neurons at multiple ages
    Authors: Chao Li, Xiang Li, Zhenghong Bi, Ken Sugino, Guangqin Wang, Tong Zhu et al.
    eLife
  40. Dynamic Expression of Sox2, Gata3, and Prox1 during Primary Auditory Neuron Development in the Mammalian Cochlea
    Authors: Koji Nishimura, Teppei Noda, Alain Dabdoub
    PLOS ONE
  41. A Toolbox to Characterize Human Induced Pluripotent Stem Cell-Derived Kidney Cell Types and Organoids.
    Authors: Vanslambrouck J M, Wilson S B et al.
    J Am Soc Nephrol
  42. Early ear neuronal development, but not olfactory or lens development, can proceed without SOX2
    Authors: Martina Dvorakova, Iva Macova, Romana Bohuslavova, Miroslava Anderova, Bernd Fritzsch, Gabriela Pavlinkova
    Developmental Biology
  43. Predicting response to bacillus Calmette-Guérin (BCG) in patients with carcinoma in situ of the bladder
    Authors: Rafael Nunez-Nateras, Erik P. Castle, Cheryl A. Protheroe, Melissa L. Stanton, Tolgay I. Ocal, Erin N. Ferrigni et al.
    Urologic Oncology: Seminars and Original Investigations

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Human GATA-3 Antibody
By Alice Jouneau on 08/09/2022
Application: Immunocytochemistry/Immunofluorescence Sample Tested: blastocyst Species: Bovine

use at 10 µg/ml

Immunocytochemistry/Immunofluorescence Human GATA-3 Antibody AF2605