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Human GPRC5A Antibody

Catalog #: MAB5239
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB5239
MAB5239-SP
Detection of GPRC5A in hGPRC5A HEK293/eGFP transfectants by Flow Cytometry.
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Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GPRC5A Antibody Summary

Species Reactivity
Human
Specificity
Stains human GPRC5A transfectants but not irrelevant transfectants.
Source
Monoclonal Mouse IgG2A Clone # 481906
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human GPRC5A
Met1-Ser357
Accession # Q8NFJ5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
HEK293 human embryonic kidney cell line transfected with human GPRC5A and eGFP
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of GPRC5A antibody in A549 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of GPRC5A in hGPRC5A HEK293/eGFP transfectants by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) Human GPRC5A or (B) irrelevant protein and eGFP, was stained with Mouse Anti-Human GPRC5A Monoclonal Antibody (Catalog # MAB5239) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on isotype control antibody (MAB003). Staining was performed using our Staining Membrane-Associated Proteins protocol.

Intracellular Staining by Flow Cytometry Detection of GPRC5A antibody in A549 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of GPRC5A in A549 Human Cell Line by Flow Cytometry. A549 human lung carcinoma cell line was stained with Mouse Anti-Human GPRC5A Monoclonal Antibody (Catalog # MAB5239, filled histogram) or isotype control antibody (MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPRC5A

G-protein coupled receptor family C group 5 member A (GPRC5A), also known as Retinoic acid-induced gene 1 protein (RAIG-1), is a member of the G-protein coupled receptor 3 family. It has a molecular weight of approximately 35 kDa and contains seven transmembrane domains. Human GPRC5A shares 76% and 74% aa sequence identity with mouse and rat GPRC5A, respectively. It is expressed at high levels in fetal and adult lung and is also present in fetal kidney and adult placenta, kidney, prostate, testis, ovary, small intestine, colon, stomach, and spinal chord at lower levels.

Long Name
G Protein-coupled Receptor, Family C, Group 5, Member A
Entrez Gene IDs
9052 (Human); 232431 (Mouse); 312790 (Rat)
Alternate Names
G protein-coupled receptor, family C, group 5, member A; GPCR5A; GPRC5A; Orphan G-protein-coupling receptor PEIG-1; RAI3; RAI3G-protein coupled receptor family C group 5 member A; RAIG1; RAIG1RAIG-1; retinoic acid induced 3; retinoic acid responsive; Retinoic acid-induced gene 1 protein; retinoic acid-induced protein 3

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Citation for Human GPRC5A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Quantitative proteomics of MDCK cells identify unrecognized roles of clathrin adaptor AP-1 in polarized distribution of surface proteins
    Authors: PS Caceres, D Gravotta, PJ Zager, N Dephoure, E Rodriguez-
    Proc. Natl. Acad. Sci. U.S.A., 2019-05-29;116(24):11796-11805.
    Species: Canine
    Sample Types: Cell Lysates
    Applications: Western Blot

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