Human HCN4 Antibody

Catalog #: MAB8138
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8138
MAB8138-SP

HCN4 in BG01V Human Embryonic Stem Cells.

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All HCN4 Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human HCN4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human HCN4 in ELISA

Source

Monoclonal Mouse IgG₁ Clone # 880904

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human HCN4
Lys1084-Leu1203
Accession # Q9Y3Q4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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HCN4 in BG01V Human Embryonic Stem Cells. HCN4 was detected in immersion fixed BG01V human embryonic stem cells differentiated to cardiac cells using Mouse Anti-Human HCN4 Monoclonal Antibody (Catalog # MAB8138) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HCN4

HCN4 (hyperpolarization-activated cyclic nucleotide-gated channel 4) is a 120-160 kDa, 1203 amino acid (aa) multipass plasma membrane protein that is a member of the HCN family of potassium channel proteins. It is mainly expressed in cardiac myocytes, thalamus and testis. HCN4 is important for pacemaker function in the heart, and rhythmic activity in the thalamus. Specific mutations can cause Sick Sinus Syndrome 2 (SSS2), a cardiac sinus node malfunction, or Brugada syndrome 8 (BRGDA8), a tachyarrhythmia syndrome. Within aa 1084-1203, human HCN4 shares 92% and 93% aa sequence identity with mouse and rat HCN4, respectively.

Long Name

Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 4

Entrez Gene IDs

10021 (Human); 330953 (Mouse); 59266 (Rat)

Alternate Names

HCN4; hyperpolarization activated cyclic nucleotide-gated cation channel 4; hyperpolarization activated cyclic nucleotide-gated potassium channel 4; potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4; SSS2

Product Datasheets

Product Datasheet

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Related Research Areas

Citation for Human HCN4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1