Human HES-1 Antibody

Catalog #: AF3317
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3317
AF3317-SP

Detection of Human HES‑1 by Western Blot.

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All HES-1 Antibodies

Product Details

Citations (4)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human HES-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human HES-1 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human (rh) HES‑2, rhHES-4, rhHES-5, rhHES-6, and rhHES-7 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human HES-1
Arg93-Asn280
Accession # Q14469

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human HES‑1 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human HES-1. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human HES-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3317) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for HES-1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry

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HES‑1 in Human Ovarian Cancer Tissue. HES-1 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Goat Anti-Human HES-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3317) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HES-1

HES-1 is a transcriptional repressor that is a target of notch signaling. It contains a basic helix-loop-helix (bHLH) DNA-binding domain, an Orange domain and a C‑terminal tetrapeptide WRPW motif that binds to the Groucho (Gro)/TLE/Grg family of corepressors. HES-1 can form both homo- and heterodimers with other HES family members. Dimerization is mediated through both the bHLH and the downstream Orange domain. Over the sequence used for immunization, human HES-1 shares 97% and 98% amino acid sequence identity with mouse and rat HES-1, respectively.

Long Name

Hairy And Enhancer of Split 1

Entrez Gene IDs

3280 (Human); 15205 (Mouse); 29577 (Rat)

Alternate Names

BHLHB39; bHLHb39hairy homolog (Drosophila); Class B basic helix-loop-helix protein 39; FLJ20408; Hairy and enhancer of split 1; hairy and enhancer of split 1, (Drosophila); Hairy homolog; Hairy-like protein; HES1; HES-1; hHL; HL; HRY; HRYHHL; transcription factor HES-1

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Human HES-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Density of human bone marrow stromal cells regulates commitment to vascular lineages
Authors: Jemima L. Whyte, Stephen G. Ball, C. Adrian Shuttleworth, Keith Brennan, Cay M. Kielty
Stem Cell Research
Decreased p53 is associated with a decline in asymmetric stem cell self-renewal in aged human epidermis
Authors: A Charruyer, T Weisenberg, H Li, A Khalifa, AW Schroeder, A Belzer, R Ghadially
Aging Cell, 2021-02-01;0(0):e13310.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Interplay between CCR7 and Notch1 axes promotes stemness in MMTV-PyMT mammary cancer cells
Authors: ST Boyle, KA Gieniec, CE Gregor, JW Faulkner, SR McColl, M Kochetkova
Mol. Cancer, 2017-01-31;16(1):19.
Species: Mouse
Sample Types: Whole Cells
Applications: Western Blot
Rabconnectin-3 is a functional regulator of mammalian Notch signaling.
Authors: Sethi N, Yan Y, Quek D
J. Biol. Chem., 2010-09-01;285(45):34757-64.
Species: Human, Mouse
Sample Types: Whole Cells
Applications: ICC