Human ICAM-5 Antibody Summary
Ala28-Glu570
Accession # Q9UMF0
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human ICAM‑5 by Western Blot. Western blot shows lysates of human brain (motor cortex) tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human ICAM-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1950) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for ICAM-5 at approximately 115 and 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human ICAM‑5 by Simple WesternTM. Simple Western lane view shows lysates of human brain (motor cortex) tissue, loaded at 0.2 mg/mL. Specific bands were detected for ICAM-5 at approximately 146 and 180 kDa (as indicated) using 1 µg/mL of Goat Anti-Human ICAM-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1950) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ICAM-5
Intercellular adhesion molecule-5 (ICAM-5), also known as telencephalin, is a cell surface glycoprotein belonging to the immunoglobulin superfamily. Human ICAM-5 consists of an 832 amino acid (aa) extracellular domain containing 9 immunoglobulin (Ig) domains and 15 N-glycosylation sites, a 28 aa transmembrane domain, and a 64 aa cytoplasmic domain. ICAM-5 shares 38‑55% aa identity with other ICAMs, being most closely related to ICAM-1 (50% identity) and ICAM-3 (55% identity) (1). Human and mouse ICAM-5 share 85% aa identity. The tissue distribution of ICAM-5 is unique among ICAMs, being expressed only in telencephalic regions of the central nervous system (2). Like other ICAMs, ICAM-5 binds to the leukocyte integrin LFA-1 (CD11a/CD18) (3). Binding of ICAM-5 to LFA-1 is dependent on the first amino terminal Ig domain of ICAM-5 (4). ICAM-5 also displays homophilic binding, with the amino terminal Ig domain binding to Ig domains 4‑5. Homophilic binding of ICAM-5 is dependent of ICAM-5 being in a monomeric form. The monomeric form of ICAM-5 is found during dendritogenesis in developing brain, whereas a high molecular weight complex is found in mature neurons (5).
- Mizuno, T. et al. (1997) J. Biol. Chem. 272:1156.
- Yoshihara, Y. et al. (1994) Neuron 12:541.
- Tain, L. et al. (1997) J. Immunol. 158:928.
- Tain, L. et al. (2000) Eur. J. Immunol. 30:810.
- Tain, L. et al. (2000) J. Immunol. 150:243.
Product Datasheets
Citations for Human ICAM-5 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Multivariate analysis of traumatic brain injury: development of an assessment score.
Authors: Buonora JE, Yarnell AM, Lazarus RC et al.
Front Neurol
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ICAM-5/Telencephalin Is a Functional Entry Receptor for Enterovirus D68
Authors: Wei Wei
Cell Host Microbe, 2016-10-27;20(5):631-641.
Applications: Flow Cytometry, Western Blot
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