Human ID2 Antibody

Catalog #: AF4660 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4660
AF4660-SP

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Human ID2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ID2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) ID1, rhID3, and rhID4 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human ID2
Met1-Gly134
Accession # Q02363

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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ID2 in A549 Human Cell Line. ID2 was detected in immersion fixed A549 human lung carcinoma cell line using 10 µg/mL Human ID2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4660) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Western Blot

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Detection of Human ID2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human ID2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4660) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ID2 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ID2

ID2 (Inhibitor of DNA binding 2; also known as inhibitor of differentiation 2) is a 15 kDa member of the small ID HLH family of proteins. It is a nuclear protein that forms inactivating heterodimers with bHLH transcription factors. bHLH proteins activate lineage-specific genes and ID2. Human ID2 is 134 amino acids (aa) in length. It contains one HLH domain (aa 36-76) but no DNA-binding motif. Full-length human ID2 is 98% aa identical to both mouse and porcine ID2.

Long Name

Inhibitor of DNA Binding 2

Entrez Gene IDs

3398 (Human); 15902 (Mouse); 25587 (Rat)

Alternate Names

BHLHB26; bHLHb26cell growth-inhibiting gene 8; Class B basic helix-loop-helix protein 26; DNA-binding protein inhibitor ID2; DNA-binding protein inhibitor ID-2; GIG8; helix-loop-helix protein ID2; ID2; ID2A; ID2H; inhibitor of differentiation 2; Inhibitor of DNA binding 2; inhibitor of DNA binding 2, dominant negative helix-loop-helix protein; MGC26389