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Human IFITM2/IFITM3 Antibody

Catalog #: AF4834
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4834
AF4834-SP
Detection of Human IFITM2/IFITM3/Fragilis by Western Blot.
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Citations (3)
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Human IFITM2/IFITM3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IFITM2 and human IFITM3 in direct ELISAs and Western blots. In direct ELISAs, less than 4% cross-reactivity with recombinant human IFITM1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived human IFITM3
His3-His57
Accession # Q01628
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human IFITM2/IFITM3/Fragilis antibody by Western Blot. View Larger

Detection of Human IFITM2/IFITM3/Fragilis by Western Blot. Western blot shows lysates of human brain tissue and human spleen tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IFITM2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4834) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for IFITM2 and IFITM3 at approximately 15 and 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFITM2/IFITM3

IFITM3 (IFN-inducible tranemembrane protein 3; also IFN-inducible protein 1-8U and fragilis) is a 14.6 kDa (predicted) member of the CD225 family of proteins. It is expressed on primordial germ cells and select tumor types, and appears to regulate cell migration. Human IFITM3 is a 133 amino acid (aa), two-transmembrene protein that contains an N-terminal (aa 1-57) and a C-terminal (aa 108-128) extracellular domain. Over aa 3-57, human IFITM3 is 59% and 56% aa identical to bovine and mouse IFITM3, respectively. Over aa 3-57 human IFITM3 is 88% identical to human IFITM2.

Alternate Names
1-8D;1-8U;DSPA2b;DSPA2c;IFITM2;IFITM3;IP15; IFITM2/IFITM3

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Citations for Human IFITM2/IFITM3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. IFITM3 restricts virus-induced inflammatory cytokine production by limiting Nogo-B mediated TLR responses
    Authors: M Clement, JL Forbester, M Marsden, P Sabberwal, MS Sommervill, D Wellington, S Dimonte, S Clare, K Harcourt, Z Yin, L Nobre, R Antrobus, B Jin, M Chen, S Makvandi-N, JA Lindborg, SM Strittmatt, MP Weekes, RJ Stanton, T Dong, IR Humphreys
    Nature Communications, 2022-09-08;13(1):5294.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. ?20 IFITM2 differentially restricts X4 and R5 HIV-1
    Authors: WL Wu, CR Grotefend, MT Tsai, YL Wang, V Radic, H Eoh, IC Huang
    Proc. Natl. Acad. Sci. U.S.A., 2017-06-19;114(27):7112-7117.
    Species: Human
    Sample Types: Recombinant Protein, Whole Cells
    Applications: ICC, Western Blot
  3. IFITM3 Polymorphism rs12252-C Restricts Influenza A Viruses
    Authors: David Evan Joseph Williams, Wan-Lin Wu, Christopher Robert Grotefend, Vladimir Radic, Changik Chung, Young-Hwa Chung et al.
    PLoS ONE

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