Human IGFBP-5 Antibody Summary
Glu28-Glu272
Accession # P24593
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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IGFBP‑5 in Human Placenta. IGFBP-5 was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human IGFBP-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF875) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblasts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of Human IGFBP-5 by Immunohistochemistry Immunoreactivity (red colour) against IGFBP-4, −5, and PAPPA2 in the syncytiotrophoblast of first trimester placental villi. Serial cross sections of placental villi at 5 and 6 weeks of gestation (7 and 13 weeks not shown) were stained for IGFBP-4, IGFBP-5, and PAPP-A2. Negative controls used non-specific goat IgG in the place of the primary antibodies. FB = fetal blood vessel, CM = chorionic mesoderm, ST = syncytiotrophoblast. Scale bars denote 100 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25475528), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Human IGFBP-5 by Immunohistochemistry Immunoreactivity (red colour) against IGFBP-4, −5, and PAPPA2 in the syncytiotrophoblast of first trimester placental villi. Serial cross sections of placental villi at 5 and 6 weeks of gestation (7 and 13 weeks not shown) were stained for IGFBP-4, IGFBP-5, and PAPP-A2. Negative controls used non-specific goat IgG in the place of the primary antibodies. FB = fetal blood vessel, CM = chorionic mesoderm, ST = syncytiotrophoblast. Scale bars denote 100 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25475528), licensed under a CC-BY license. Not internally tested by R&D Systems.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IGFBP-5
The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-transitional modifications of IGFBP, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF.
Human IGFBP-5 cDNA encodes a 272 amino acid (aa) residue precursor protein with a putative 20 aa residue signal peptide that is processed to generate the 252 aa residue mature protein that is post-translationally modified by O-glycosylations and serine phosphorylations. IGFBP-5 is expressed by fibroblasts, myoblasts and osteoblasts, making it the predominant IGFBP found in bone extracts. IGFBP-5 has a strong affinity for hydroxyapatite, allowing it to bind to bone cells. When bound to extracellular matrix, IGFBP-5 is protected from proteolysis and potentiates IGF activity, but when it is soluble, IGFBP-5 is cleaved to a biologically inactive 21 kDa fragment (1, 2).
- Jones, J.I. and D.R. Clemmons (1995) Endocrine Rev. 16:3.
- Kelley, K.M. et al. (1996) Int. J. Biochem. Cell Biol. 28:619.
Product Datasheets
Citations for Human IGFBP-5 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
9
Citations: Showing 1 - 9
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Placental transcriptomes in the common aneuploidies reveal critical regions on the trisomic chromosomes and genome-wide effects Prenat.
Authors: Bianco K, Gormley M, Farrell J et al.
Diagn.
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IGF-binding proteins secreted by cancer-associated fibroblasts induce context-dependent drug sensitization of lung cancer cells
Authors: Lily L. Remsing Remsing Rix, Natalia J. Sumi, Qianqian Hu, Bina Desai, Annamarie T. Bryant, Xueli Li et al.
Science Signaling
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Insulin-like growth factor 5 associates with human A� plaques and promotes cognitive impairment
Authors: S Rauskolb, T Andreska, S Fries, CR von Collen, R Blum, CM Monoranu, C Villmann, M Sendtner
Acta neuropathologica communications, 2022-05-05;10(1):68.
Species: Human, Mouse
Sample Types: Whole Tissue
Applications: IHC -
Netazepide Inhibits Expression of Pappalysin 2 in Type-1 Gastric Neuroendocrine Tumors
Authors: KA Lloyd, BN Parsons, MD Burkitt, AR Moore, S Papoutsopo, M Boyce, CA Duckworth, K Exarchou, N Howes, L Rainbow, Y Fang, C Oxvig, S Dodd, A Varro, N Hall, DM Pritchard
Cell Mol Gastroenterol Hepatol, 2020-01-28;0(0):.
Species: Human
Sample Types: Cell Cultue Supernates
Applications: Western Blot -
IGFBP-4 and -5 are expressed in first-trimester villi and differentially regulate the migration of HTR-8/SVneo cells.
Authors: Crosley, Erin J, Dunk, Caroline, Beristain, Alexande, Christians, Julian K
Reprod Biol Endocrinol, 2014-12-04;12(0):123.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Increased levels of pregnancy-associated plasma protein-A2 in the serum of pre-eclamptic patients.
Authors: Nishizawa H, Pryor-Koishi K, Suzuki M
Mol. Hum. Reprod., 2008-09-18;14(10):595-602.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
The role of matrix metalloproteinase-7 in redefining the gastric microenvironment in response to Helicobacter pylori.
Authors: McCaig C, Duval C, Hemers E, Steele I, Pritchard DM, Przemeck S, Dimaline R, Ahmed S, Bodger K, Kerrigan DD, Wang TC, Dockray GJ, Varro A
Gastroenterology, 2006-03-06;130(6):1754-63.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Matrix metalloproteinase-7 degrades all insulin-like growth factor binding proteins and facilitates insulin-like growth factor bioavailability.
Authors: Nakamura M, Miyamoto S, Maeda H, Ishii G, Hasebe T, Chiba T, Asaka M, Ochiai A
Biochem. Biophys. Res. Commun., 2005-08-05;333(3):1011-6.
Species: Human
Sample Types: Recombinant Protein
Applications: Western Blot -
IGFBP-5 Metabolism Is Disrupted in the Rat Medial Meniscal Tear Model of Osteoarthritis
Authors: Matthew P. Yates, Steven L. Settle, Sue A. Yocum, Poonam Aggarwal, Lillian E. Vickery, Dean J. Aguiar et al.
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