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Human IGFBP-L1 Antibody

Catalog #: AF3877 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3877
AF3877-SP
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IGFBP-L1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IGFBP-L1 in direct ELISAs and Western blots. In Western blots, approximately 15% cross-reactivity with recombinant mouse (rm) IGFBP-L1 is observed and less than 1% cross-reactivity with recombinant human IGFBP-1, -2, -3, -4, -5, -6, -7, and rmIGFBP-rP10 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IGFBP-L1
Ser25-Met278
Accession # Q8WX77
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IGFBP-L1

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGFBP-L1

IGFBP-L1 is a secreted 38 kDa protein that shares 43% amino acid sequence identity with IGFBP-7 (IGFBP-rP1, Mac25). Although it was also referred to as IGFBP‑rP4 in one publication, it is distinct from Cyr61, a CCN family protein also named IGFBP-rP4 at one time. IGFBP-L1 contains an N-terminal IGFBP motif, a Kazal‑type serine protease inhibitor region and a C-terminal Ig-like domain. Human and mouse mature IGFBP-L1 share 76% amino acid sequence homology.

Long Name
Insulin-like Growth Factor Binding Protein-like 1
Entrez Gene IDs
347252 (Human); 75426 (Mouse)
Alternate Names
bA113O24.1; IGFBPL1 insulin-like growth factor binding protein-like 1; IGFBPL1; IGFBP-L1; IGFBP-RP4

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