Human IL-1 RI Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of IL‑1 RI in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were labeled with Human IL-1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF269, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
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CXCL1/GRO alpha Secretion Induced by IL‑1 beta /IL‑1F2 and Neutralization by Human IL‑1 RI Antibody. Recombinant Human IL-1 beta /IL-1F2 (Catalog # 201-LB) induces CXCL1/GROa secretion in the HT-29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line),as measured by the Human CXCL1/GROa DuoSet (Catalog # DY275). CXCL1/GROa secretion elicited by Recombinant Human IL-1 beta /IL-1F2 is neutralized (green line) by increasing concentrations of Human IL-1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF269). The ND50 is typically < 5 µg/mL in the presence of 400 pg/mL Recombinant Human IL-1 beta /IL-1F2.
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Detection of Human IL-1 RI by Immunocytochemistry/ Immunofluorescence Expression of IL1RI in native MSC and inhibition of the IL1beta mediated regulation of gene expression in PTC or IL1beta stimulated MSC.A) MSC on glass slides were stained with an anti-IL1RI antibody (left) or the non-specific IgG isotype control (right). Gene expression levels of B) PTGES, C) TSG6 and F) MMP1 were measured with quantitative real-time PCR and normalized to those of HPRT1 following total RNA isolation 24 h post stimulation. D) PGE2 and E) TSG6 levels in supernatants were detected with the Biotrend PGE2 Enzyme Immunoassay Kit and TSG6 ELISA. MSC were seeded at 5.2*103 cells/cm2 and stimulated with the PTC or IL1beta after the anti-IL1RI antibody or the non-specific (ns) IgG isotype control had been added. Scatter plots and mean from up to 4 donors are presented. Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0116772), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Human IL-1 RI by Immunocytochemistry/ Immunofluorescence Expression of IL1RI in native MSC and inhibition of the IL1beta mediated regulation of gene expression in PTC or IL1beta stimulated MSC.A) MSC on glass slides were stained with an anti-IL1RI antibody (left) or the non-specific IgG isotype control (right). Gene expression levels of B) PTGES, C) TSG6 and F) MMP1 were measured with quantitative real-time PCR and normalized to those of HPRT1 following total RNA isolation 24 h post stimulation. D) PGE2 and E) TSG6 levels in supernatants were detected with the Biotrend PGE2 Enzyme Immunoassay Kit and TSG6 ELISA. MSC were seeded at 5.2*103 cells/cm2 and stimulated with the PTC or IL1beta after the anti-IL1RI antibody or the non-specific (ns) IgG isotype control had been added. Scatter plots and mean from up to 4 donors are presented. Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0116772), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 RI
Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor Type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts, and endothelial cells. IL-1 receptor Type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence identity in their extracellular domains. The two receptor types do not heterodimerize into a receptor complex.
An IL-1 receptor accessory protein that can heterodimerize with the Type I receptor in the presence of IL-1 alpha or IL-1 beta but not IL-1ra, was identified (1). This Type I receptor complex appears to mediate all the known IL-1 biological responses. The receptor Type II has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the Type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling Type I receptor complex. Recombinant IL-1 soluble receptor Type I is a potent antagonist of IL-1 action.
Product Datasheets
Citations for Human IL-1 RI Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
12
Citations: Showing 1 - 10
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Cancer genes disfavoring T�cell immunity identified via integrated systems approach
Authors: RJ Kishton, SJ Patel, AE Decker, SK Vodnala, M Cam, TN Yamamoto, Y Patel, M Sukumar, Z Yu, M Ji, AN Henning, D Gurusamy, DC Palmer, RA Stefanescu, AT Girvin, W Lo, A Pasetto, P Malekzadeh, DC Deniger, KC Wood, NE Sanjana, NP Restifo
Cell Reports, 2022-08-02;40(5):111153.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Naive and memory CD4+ T cell subsets can contribute to the generation of human Tfh cells
Authors: Raphaël Jeger-Madiot, Romain Vaineau, Maud Heredia, Nicolas Tchitchek, Lisa Bertrand, Mathias Pereira et al.
iScience
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Interleukin-1 beta Induces mtDNA Release to Activate Innate Immune Signaling via cGAS-STING
Authors: Lauren D. Aarreberg, Katharina Esser-Nobis, Connor Driscoll, Andrey Shuvarikov, Justin A. Roby, Michael Gale
Molecular Cell
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Expression and function of IL-1R8 (TIR8/SIGIRR), a regulatory member of the IL-1 receptor family in platelets
Authors: Achille Anselmo
Cardiovasc Res, 2016-06-13;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Priming of Human Resting NK Cells by Autologous M1 Macrophages via the Engagement of IL-1beta, IFN-beta, and IL-15 Pathways.
Authors: Mattiola I, Pesant M, Tentorio P, Molgora M, Marcenaro E, Lugli E, Locati M, Mavilio D
J Immunol, 2015-08-14;195(6):2818-28.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Crucial role of IL1beta and C3a in the in vitro-response of multipotent mesenchymal stromal cells to inflammatory mediators of polytrauma.
Authors: Hengartner N, Fiedler J, Schrezenmeier H, Huber-Lang M, Brenner R
PLoS ONE, 2015-01-06;10(1):e0116772.
Species: Human
Sample Types: Whole Cells
Applications: Blocking, ICC -
IL-1beta promotes the differentiation of polyfunctional human CCR6+CXCR3+ Th1/17 cells that are specific for pathogenic and commensal microbes.
Authors: Duhen T, Campbell D
J Immunol, 2014-06-02;193(1):120-9.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Interleukin-33 Drives a Proinflammatory Endothelial Activation That Selectively Targets Nonquiescent Cells
Authors: Jürgen Pollheimer, Johanna Bodin, Olav Sundnes, Reidunn J. Edelmann, Sigrid S. Skånland, Jon Sponheim et al.
Arteriosclerosis, Thrombosis, and Vascular Biology
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Pathogen-induced human TH17 cells produce IFN-gamma or IL-10 and are regulated by IL-1beta.
Authors: Zielinski CE, Mele F, Aschenbrenner D, Jarrossay D, Ronchi F, Gattorno M, Monticelli S, Lanzavecchia A, Sallusto F
Nature, 2012-04-26;484(7395):514-8.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Staphylococcus aureus stimulates neutrophil targeting chemokine expression in keratinocytes through an autocrine IL-1alpha signaling loop
Authors: Florina Olaru, Liselotte E. Jensen
Journal of Investigative Dermatology
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Chorionic gonadotropin down-regulates the expression of the decoy inhibitory interleukin 1 receptor type II in human endometrial epithelial cells.
Authors: Herrmann-Lavoie C, Rao CV, Akoum A
Endocrinology, 2007-08-16;148(11):5377-84.
Species: Human
Sample Types: Cell Culture Supernates, Cell Lysates
Applications: ELISA Development, Western Blot -
Interleukin 1 in oviductal tissues of viviparous, oviparous, and ovuliparous species of amphibians.
Authors: Jantra S, Bigliardi E, Brizzi R, Ietta F, Bechi N, Paulesu L
Biol. Reprod., 2007-02-21;76(6):1009-15.
Species: Amphibian
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-P, Western Blot
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This polyclonal antibody (AF269) was used as both capture and detection for a sandwich assay for sIL-1R1. The immunoassay standard used was recombinant IL-1R1, 269-1R.
