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Human IL-15R alpha Antibody

Catalog #: AF247
25 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF247
AF247-SP
IL‑15 R alpha in HDLM‑2 Human Cell Line.
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Product Details
Citations (25)
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Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-15R alpha Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-15 R alpha in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human
IL‑2 R gamma is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human
IL‑15 R alpha
Ile31-Thr172
Accession # EAW86418
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IL‑15 R alpha Fc Chimera (Catalog # 147-IR)
Flow Cytometry
2.5 µg/106 cells
Human peripheral blood mononuclear cells treated with PHA
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Dual RNAscope ISH-IHC
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human pancreas
Immunocytochemistry
1-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize IL‑15 R alpha -mediated inhibition of proliferation in the CTLL‑2 mouse cytotoxic T cell line. The Neutralization Dose (ND50) is typically 1-4 µg/mL in the presence of 30 ng/mL Recombinant Human IL‑15 R alpha Fc Chimera and 2 ng/mL Recombinant Human IL‑15.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry IL-15 Ra antibody in HDLM-2 Human Cell Line by Immunocytochemistry (ICC). View Larger

IL‑15 R alpha in HDLM‑2 Human Cell Line. IL-15 Ra was detected in immersion fixed HDLM-2 human Hodgkin's lymphoma cell line using Goat Anti-Human IL-15 Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF247) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry IL‑15 Ra antibody in Human Prostate Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

IL‑15 R alpha in Human Prostate Cancer Tissue. IL‑15 Ra was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 15 µg/mL Goat Anti-Human IL‑15 Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF247) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Neutralization IL‑15 R alpha Inhibition of IL‑15-dependent Cell Proliferation and Neutralization by Human IL‑15 R alpha Antibody. View Larger

IL‑15 R alpha Inhibition of IL‑15-dependent Cell Proliferation and Neutralization by Human IL‑15 R alpha Antibody. Recombinant Human IL-15 Ra Fc Chimera (Catalog # 147-IR) inhibits Recombinant Human IL-15 (Catalog # 247-IL) induced proliferation in the CTLL-2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-15 (2 ng/mL) activity elicited by Recombinant Human IL-15 Ra Fc Chimera (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-15 Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF247). The ND50 is typically 1-4 µg/mL.

ELISA Detection of Human IL-15R alpha by ELISA View Larger

Detection of Human IL-15R alpha by ELISA Measured levels of sIL-15Ralpha, IL-15, IL-6 and TNFalpha in synovial fluids from RA and OA patients. Cytokine concentration of IL-15Ralpha (A), IL-15 (B), TNFalpha (C) and IL-6 (D) were measured in in duplicate SF samples from RA (n = 30) and OA (n = 30) by ELISA. Box plot represents media ± SD. Differences between two groups were performed by Mann–Whitney U test for nonparametric data. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25879761), licensed under a CC-BY license. Not internally tested by R&D Systems.

In-situ Hybridization View Larger

Detection of IL-15R alpha in Human Pancreas. Formalin-fixed paraffin-embedded tissue sections of human pancreas were probed for IL15Ra mRNA (ACD RNAScope Probe, catalog #532368; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using goat anti-human IL15Ra polyclonal antibody (R&D Systems catalog # AF247) at 3ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to exocrine glands.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-15R alpha

Interleukin 15 receptor alpha (IL-15 R alpha ) is a high affinity receptor that specifically binds IL-15 with high affinity and associates as a heterotrimer with the IL-2 receptors beta and gamma subunits to initiate signal transduction. IL-15 R alpha is expressed on a wide variety of T cells and B cells as well as non-lymphoid cells.
IL‑15 R alpha is a 58-60 kDa protein that shares structural similarities to the IL-2 R alpha protein. IL-15 R alpha and IL-2 R alpha genes also share similar intron-exon organization and are closely linked on human chromosome 10p14-p15. Human IL-15 R alpha shares 45% amino acid (aa) homology with the mouse form of the receptor. Eight isoforms of
IL‑15 R alpha mRNA have been identified resulting from alternative splicing events involving different exons. The exclusion of exon 2 results in an IL-15 R alpha isoform that does not bind IL-15. Human IL-15 R alpha DE3 cDNA encodes a 267 amino acid (aa) protein that contains a 30 aa signal sequence, a 175 aa extracellular region containing one N-linked glycosylation site, a 21 aa transmembrane domain, and a 41 aa cytoplasmic tail. Signaling of IL-15 can occur in one of three ways; through the heterotrimeric complex of IL-15 R alpha, IL-2 R beta, and IL-2 R gamma c, through the heterodimeric complex of IL-2 receptors beta and gamma common, through a novel 60-65 kDa IL-15 RX subunit found on mast cells. The binding of IL-15 to IL-15 R alpha has been reported to antagonize the TNF-alpha -mediated apoptosis in fibroblasts by competing with TNF RI for TRAF2 binding.

References
  1. Anderson, D.M. et al. (1995) J. Biol. Chem. 270:29862.
  2. Bulfone-Paus, S. et al. (1999) FASEB 13:1575.
  3. Waldemann, T.A. and Y. Tagaya (1999) Ann. Rev. Immunol. 17:19.
  4. Dubois, S. et al. (1999) J. Biol. Chem. 274:26978.
Long Name
Interleukin 15 Receptor alpha
Entrez Gene IDs
3601 (Human); 16169 (Mouse); 102121571 (Cynomolgus Monkey)
Alternate Names
CD215 antigen; CD215; IL15 R alpha; IL-15 R alpha; IL-15 receptor subunit alpha; IL-15R alpha; IL15RA; IL-15Ra; IL-15R-alpha; interleukin 15 receptor, alpha; interleukin-15 receptor subunit alpha; MGC104179

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Citations for Human IL-15R alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

25 Citations: Showing 1 - 10
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  1. Myeloid antigen-presenting cell niches sustain antitumor T cells and license PD-1 blockade via CD28 costimulation
    Authors: Jaikumar Duraiswamy, Riccardo Turrini, Aspram Minasyan, David Barras, Isaac Crespo, Alizée J. Grimm et al.
    Cancer Cell
  2. Complement-activated interferon-gamma –primed human endothelium transpresents interleukin-15 to CD8+ T cells
    Authors: Catherine B. Xie, Bo Jiang, Lingfeng Qin, George Tellides, Nancy C. Kirkiles-Smith, Dan Jane-wit et al.
    Journal of Clinical Investigation
  3. IL-15R alpha membrane anchorage in either cis or trans is required for stabilization of IL-15 and optimal signaling
    Authors: Agnès Quéméner, Sébastien Morisseau, Rui P. Sousa, Kilian Trillet, Mike Maillasson, Isabelle Leray et al.
    Journal of Cell Science
  4. Lymphocyte‑derived microparticles stimulate osteoclastogenesis by inducing RANKL in fibroblasts of odontogenic keratocysts
    Authors: Qi‑Wen Man, Lin‑Zhou Zhang, Yi Zhao, Jin‑Yuan Liu, Yue‑Yu Zheng, Yi‑Fang Zhao et al.
    Oncology Reports
  5. Efficient production and enhanced tumor delivery of engineered extracellular vesicles
    Authors: Dionysios C. Watson, Defne Bayik, Avinash Srivatsan, Cristina Bergamaschi, Antonio Valentin, Gang Niu et al.
    Biomaterials
  6. Recombinant human heterodimeric IL-15 complex displays extensive and reproducible N- and O-linked glycosylation
    Authors: M. Thaysen-Andersen, E. Chertova, C. Bergamaschi, E. S. X. Moh, O. Chertov, J. Roser et al.
    Glycoconjugate Journal
  7. Differential expression of pro-inflammatory cytokines IL-15Ralpha, IL-15, IL-6 and TNFalpha in synovial fluid from Rheumatoid arthritis patients
    Authors: Alicia Santos Savio, Ana Cecilia Machado Diaz, Araceli Chico Capote, Jamilet Miranda Navarro, Yunier Rodríguez Alvarez, Ricardo Bringas Pérez et al.
    BMC Musculoskeletal Disorders
  8. Priming of Human Resting NK Cells by Autologous M1 Macrophages via the Engagement of IL-1beta, IFN-beta, and IL-15 Pathways.
    Authors: Mattiola I, Pesant M, Tentorio P, Molgora M, Marcenaro E, Lugli E, Locati M, Mavilio D
    J Immunol, 2015-08-14;195(6):2818-28.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC, Neutralization
  9. IL-12–producing monocytes and HLA-E control HCMV-driven NKG2C+ NK cell expansion
    Authors: Alexander Rölle, Julia Pollmann, Eva-Maria Ewen, Vu Thuy Khanh Le, Anne Halenius, Hartmut Hengel et al.
    Journal of Clinical Investigation
  10. Intramuscular delivery of heterodimeric IL-15 DNA in macaques produces systemic levels of bioactive cytokine inducing proliferation of NK and T cells.
    Authors: Bergamaschi C, Kulkarni V, Rosati M, Alicea C, Jalah R, Chen S, Bear J, Sardesai N, Valentin A, Felber B, Pavlakis G
    Gene Ther, 2014-10-02;22(1):76-86.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Interleukin-15 enhances cellular proliferation and upregulates CNS homing molecules in pre-B acute lymphoblastic leukemia.
    Authors: Williams M, Yousafzai Y, Cox C, Blair A, Carmody R, Sai S, Chapman K, McAndrew R, Thomas A, Spence A, Gibson B, Graham G, Halsey C
    Blood, 2014-04-03;123(20):3116-27.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Regulation of CD4(+)NKG2D(+) Th1 cells in patients with metastatic melanoma treated with sorafenib: role of IL-15Ralpha and NKG2D triggering.
    Authors: Romero A, Chaput N, Poirier-Colame V, Rusakiewicz S, Jacquelot N, Chaba K, Mortier E, Jacques Y, Caillat-Zucman S, Flament C, Caignard A, Messaoudene M, Auperin A, Vielh P, Dessen P, Porta C, Mateus C, Ayyoub M, Valmori D, Eggermont A, Robert C, Zitvogel L
    Cancer Res, 2013-11-06;74(1):68-80.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  13. Paracrine and transpresentation functions of IL-15 are mediated by diverse splice versions of IL-15Ralpha in human monocytes and dendritic cells.
    Authors: Muller J, Waldmann T, Kruhlak M, Dubois S
    J Biol Chem, 2012-10-16;287(48):40328-38.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ELISA Development
  14. Proinflammatory Soluble Interleukin-15 Receptor Alpha Is Increased in Rheumatoid Arthritis
    Authors: Ana Cecilia Machado Diaz, Araceli Chico Capote, Celia Aurora Arrieta Aguero, Yunier Rodríguez Alvarez, Diana García del Barco Herrera, Miguel Estévez del Toro et al.
    Arthritis
  15. The differential production of cytokines by human Langerhans cells and dermal CD14(+) DCs controls CTL priming
    Authors: Jacques Banchereau, LuAnn Thompson-Snipes, Sandra Zurawski, Jean-Philippe Blanck, Yanying Cao, Sandra Clayton et al.
    Blood
  16. Interleukin-15 plays a central role in human kidney physiology and cancer through the gamma c signaling pathway.
    Authors: Giron-Michel J, Azzi S, Khawam K, Mortier E, Caignard A, Devocelle A, Ferrini S, Croce M, Francois H, Lecru L, Charpentier B, Chouaib S, Azzarone B, Eid P
    PLoS ONE, 2012-02-21;7(2):e31624.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  17. Increased serum soluble IL-15R alpha levels in T-cell large granular lymphocyte leukemia
    Authors: Jing Chen, Mike Petrus, Richard Bamford, Joanna H. Shih, John C. Morris, John E. Janik et al.
    Blood
  18. EBV-associated mononucleosis does not induce long-term global deficit in T-cell responsiveness to IL-15.
    Authors: Giron-Michel J, Menard F, Negrini S, Devocelle A, Azzarone B, Besson C
    Blood, 2009-03-04;113(19):4541-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. Increased IL-15 production is associated with higher susceptibility of memory CD4 T cells to simian immunodeficiency virus during acute infection.
    Authors: Eberly MD, Kader M, Hassan W, Rogers KA, Zhou J, Mueller YM, Mattapallil MJ, Piatak M, Lifson JD, Katsikis PD, Roederer M, Villinger F, Mattapallil JJ
    J. Immunol., 2009-02-01;182(3):1439-48.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. Human T cells constitutively express IL-15 that promotes ex vivo T cell homeostatic proliferation through autocrine/juxtacrine loops.
    Authors: Miranda-Carus ME, Benito-Miguel M, Llamas MA, Balsa A, Martin-Mola E
    J. Immunol., 2005-09-15;175(6):3656-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  21. Renal cells express a functional interleukin-15 receptor.
    Authors: Tejman-Yarden N, Zlotnik M, Lewis E, Etzion O, Chaimovitz C, Douvdevani A
    Nephrol. Dial. Transplant., 2005-01-25;20(3):516-23.
    Species: Human, Mouse
    Sample Types: Cell Lysates, Tissue Homogenates
    Applications: Western Blot
  22. Distinct roles of IL-12 and IL-15 in human natural killer cell activation by dendritic cells from secondary lymphoid organs.
    Authors: Ferlazzo G, Pack M, Thomas D, Paludan C, Schmid D, Strowig T, Bougras G, Muller WA, Moretta L, Munz C
    Proc. Natl. Acad. Sci. U.S.A., 2004-11-09;101(47):16606-11.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. Growth and apoptosis of human natural killer cell neoplasms: role of interleukin-2/15 signaling.
    Authors: Yamasaki S, Maeda M, Ohshima K, Kikuchi M, Otsuka T, Harada M
    Leuk. Res., 2004-10-01;28(10):1023-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  24. IL-15 and the initiation of cell contact-dependent synovial fibroblast-T lymphocyte cross-talk in rheumatoid arthritis: effect of methotrexate.
    Authors: Miranda-Carus ME, Balsa A, Benito-Miguel M, Perez de Ayala C, Martin-Mola E
    J. Immunol., 2004-07-15;173(2):1463-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  25. Cytokine profile of the rheumatoid nodule suggests that it is a Th1 granuloma.
    Authors: Hessian PA, Highton J, Kean A, Sun CK, Chin M
    Arthritis Rheum., 2003-02-01;48(2):334-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC

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Human IL-15R alpha Antibody
By Anonymous on 06/09/2020
Application: ELISA Sample Tested: Serum and Plasma Species: Human

We used this antibody in an in-house ELISA along with mAb and protein standard (147-IR-100) to quantify IL-15R alpha in human serum and plasma. This combination could not detect IL-15R alpha in our samples but generated a good standard curve.

ELISA Human IL-15R alpha Antibody AF247
Human IL-15R alpha Antibody
By Anonymous on 05/01/2020
Application: ELISA Sample Tested: Serum Species: Human

Ab was used as capture antibody in human serum ELISA

ELISA Human IL-15R alpha Antibody AF247