Human IL-18/IL-1F4 Antibody

Catalog #: D045-3
1 Citation Datasheet / COA / SDS

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D045-3

All IL-18/IL-1F4 Antibodies

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Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-18/IL-1F4 Antibody Summary

Species Reactivity

Human

Specificity

This antibody reacts with human IL-18 on Immunoprecipitation.

Source

Monoclonal Rat IgG_2A Clone # 159-12B

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human IL-18/IL-1F4

Formulation

100 μg IgG in 100 μL PBS containing 50% glycerol, pH 7.2. Contains no preservatives.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunoprecipitation

5 μg/ 0.5 μg recombinant human IL-18

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

This antibody solution is stable for one year from the date of purchase when stored at -20°C.

Background: IL-18/IL-1F4

Interleukin 18 (IL-18) is a 18 kDa cytokine which identified as a costimulatory factor for production of interferon-gamma (IFN-gamma ) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-gamma is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-gamma activates macrophages, enhances NK activity and B cell maturation, proliferation and Ig secretion, induces MHC class I and II antigens, and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-gamma. Pleiotropic effects of IL-18 has also been reported, such as, enhancement production of IFN-gamma and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-gamma in T cells, enhancement of Fas ligand expression by Th1 cells.

References

Nussbaumer, O., et al., Blood 118, 2743-2751 (2011)
Tu, A., et al., J. Exp. Med. 205, 233-244 (2008)
Wu, C., J. Immunol. 170, 5571-5577 (2003)
Sugawara, S., J. Immunol. 167, 6568-6575 (2001)
Dao, T., et al., Cell Immunol. 173, 230-235 (1996)
Micallef, M., et al., Eur. J. Immunol. 26, 1647-1651 (1996)
Ushio, S., et al., J. Immunol. 156, 4274-4279 (1996)
Okamura, H., et al., Nature 378, 88-91 (1995)

Long Name

Interleukin 18

Entrez Gene IDs

3606 (Human); 16173 (Mouse); 29197 (Rat); 397057 (Porcine); 574151 (Primate)

Alternate Names

Iboctadekin; IFN-gamma-inducing factor; IGIF; IGIFIL-1 gamma; IL18; IL-18; IL-18MGC12320; IL-1F4; IL1F4iboctadekin; IL-1g; Interferon gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor); Interleukin-1 gamma; interleukin-18

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human IL-18/IL-1F4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1