Human IL-2 R beta Antibody

Name: Human IL-2 R beta Antibody
Brand: R&D Systems
SKU: AF-224-NA
Rating: 4 (1 reviews)

Catalog #: AF-224-NA
2 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF-224-NA
AF-224-SP

Cell Proliferation Induced by IL‑2 and Neutralization by Human IL‑2 R beta Antibody.

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All IL-2 R beta Antibodies

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-2 R beta Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL‑2 R beta in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with
recombinant human (rh) IL-2 R alpha, recombinant mouse (rm) IL-2 R beta, rhIL-2 R gamma, and rhIL-15 R is observed. Is also able to block the cell surface of human IL-2 R beta mediated bioactivities induced by IL-2. For optimal neutralization of IL-2 biological activity on cells expressing the high affinity IL-2 receptors, the use of anti-IL-2 R alpha in conjunction with anti-IL-2 R beta antibodies is recommended.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑2 R beta
Ala27-Asp239
Accession # NP_000869

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human IL‑2 R beta (Catalog # 224-2B)

Simple Western

25 µg/mL

HDLM‑2 human Hodgkin's lymphoma cell line

Neutralization

Measured by its ability to neutralize IL‑2-induced proliferation in the MO7e human megakaryocytic leukemic cell line. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The Neutralization Dose (ND₅₀) is typically 10-30 µg/mL in the presence of 20 ng/mL Recombinant Human IL‑2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by IL‑2 and Neutralization by Human IL‑2 R beta Antibody. Recombinant Human IL-2 (Catalog # 202-IL) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-2 R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-224-NA). The ND₅₀ is typically 10‑30 µg/mL.

Simple Western

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Detection of Human IL‑2 R beta by Simple Western^TM. Simple Western lane view shows lysates of HDLM‑2 human Hodgkin’s lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for IL‑2 R beta at approximately 98 kDa (as indicated) using 25 µg/mL of Goat Anti-Human IL‑2 R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-224-NA). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-2 R beta

Functional IL-2 receptors can exist in two affinity states on cell surfaces, the high affinity complex consisting of heterotrimers of the alpha, beta, and gamma chains, and the intermediate affinity complex comprising heterodimers of the beta and gamma chains. Individual beta chains and alpha chains exhibit low affinity IL-2 binding and the gamma chain alone does not bind IL-2. In addition to their involvement in IL-2 mediated signal transduction, both the beta chain and gamma chain have been shown to be required for IL-15 mediated signaling.

IL-2 R beta is a member of the cytokine receptor superfamily. Human IL-2 R beta cDNA encodes a 551 amino acid residue precursor Type I membrane protein with a 26 residue signal peptide, a 214 residue extracellular region, a 25 residue transmembrane region and a 286 residue cytoplasmic domain. A soluble IL-2 R beta (IL-2 sR beta ) has been identified in the culture supernatants of a human lymphoid cell line, YT, that displays IL-2 R beta. At present, the function of IL-2 sR beta is unclear. Recombinant human IL-2 sR beta binds IL-2 with low affinity and is not an effective IL-2 antagonist on cells displaying the high or intermediate affinity IL-2 signaling receptors. Nevertheless, IL-2 sR beta binds IL-15 with sufficient affinity to neutralize IL-15 biological activities.

Long Name

Interleukin 2 Receptor beta

Entrez Gene IDs

3560 (Human); 16185 (Mouse)

Alternate Names

CD122 antigen; CD122; high affinity IL-2 receptor beta subunit; High affinity IL-2 receptor subunit beta; IL-15 R beta; IL-2 R beta; IL-2 receptor subunit beta; IL2R beta; IL-2R subunit beta; IL2RB; IL-2Rb; interleukin 15 receptor, beta; interleukin 2 receptor, beta; interleukin-2 receptor subunit beta; P70-75; p75

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Citations for Human IL-2 R beta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Late expression of granulysin by microbicidal CD4+ T cells requires PI3K- and STAT5-dependent expression of IL-2Rbeta that is defective in HIV-infected patients.
Authors: Zheng CF, Jones GJ, Shi M, Wiseman JC, Marr KJ, Berenger BM, Huston SM, Gill MJ, Krensky AM, Kubes P, Mody CH
J. Immunol., 2008-06-01;180(11):7221-9.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Growth and apoptosis of human natural killer cell neoplasms: role of interleukin-2/15 signaling.
Authors: Yamasaki S, Maeda M, Ohshima K, Kikuchi M, Otsuka T, Harada M
Leuk. Res., 2004-10-01;28(10):1023-31.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization