Human IL-5 Antibody Summary
Ile20-Ser134
Accession # P05113
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Cell Proliferation Induced by IL‑5 and Neutralization by Human IL‑5 Antibody. Recombinant Human IL‑5 (Catalog # 205-IL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑5 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-205-NA). The ND50 is typically 0.3-0.9 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-5
Interleukin 5 is a T cell-derived factor that promotes the proliferation, differentiation and activation of eosinophils. In mice, IL-5 has also been shown to be a growth and differentiation factor for B cells. Various names previously used to describe IL-5 include: T cell replacing factor (TRF), B cell growth factor II (BCGFII), B cell differentiation factor μ (BCDF μ), eosinophil differentiation factor (EDF) and eosinophil colony-stimulating factor (Eo-CSF). Biologically active IL-5 is a disulfide-linked homodimer. The cDNAs for murine and human IL-5 encode precursor proteins with signal peptides that are cleaved to form mature proteins containing 113 and 115 amino acid residues, respectively. Murine and human IL-5 are 70% identical in their amino acid sequences and show species cross‑reactivity. The genes for human and mouse IL-5 have been mapped to chromosome 5 and chromosome 11, respectively; closely linked to the genes for IL-3, IL-4 and GM-CSF.
IL-5 exerts its activity on target cells by binding to specific cell surface receptors. The functional high‑affinity receptor for human IL-5 has been shown to be composed of a low-affinity IL-5 binding alpha -subunit and a non-binding common beta -subunit that is shared with the high‑affinity receptors for GM‑CSF and IL-3.
Product Datasheets
Citations for Human IL-5 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Secretory IgA induces antigen-independent eosinophil survival and cytokine production without inducing effector functions.
Authors: Bartemes KR, Cooper KM, Drain KL, Kita H
J. Allergy Clin. Immunol., 2005-10-01;116(4):827-35.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Development and validation of a kinetic assay for analysis of anti-human interleukin-5 monoclonal antibody (SCH 55700) and human interleukin-5 interactions using surface plasmon resonance.
Authors: DiGiacomo RA, Xie L, Cullen C, Indelicato SR
Anal. Biochem., 2004-04-15;327(2):165-75.
Species: Human
Sample Types:
Applications: Neutralization -
Bleomycin stimulates lung fibroblast and epithelial cell lines to release eosinophil chemotactic activity.
Authors: Sato E, Koyama S
Eur. Respir. J., 2000-11-01;16(5):951-8.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
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