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Human Kallikrein 2 Antibody

Catalog #: MAB4104
1 Citation Datasheet / COA / SDS
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MAB4104
MAB4104-SP
Product Details
Citations (1)
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Human Kallikrein 2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Kallikrein 2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Kallikrein 1, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, or B1 is observed.
Source
Monoclonal Mouse IgG1 Clone # 426723
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Kallikrein 2
Ile25-Pro261
Accession # P20151
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Kallikrein 2 (Catalog # 4104-SE).
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Kallikrein 2 (Catalog # 4104-SE), see our available Western blot detection antibodies
Neutralization
Measured by its ability to neutralize Recombinant Human Kallikrein 2 (2 µg/mL, Catalog # 4104-SE) cleavage of the fluorogenic peptide substrate PFR-AMC (0.1 mM). The Neutralization Dose (ND50) is typically 3.4 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kallikrein 2

Kallikrein 2 (KLK2) is a secreted serine protease that is highly expressed in the human prostate gland (1). It is also known as the prostate-specific glandular kallikrein. The enzyme is highly specific for cleavage after arginine residues. KLK2 is able to activate the urokinase-type plasminogen activator (2). KLK2 is inhibited by serpins such as protein C inhibitor, antichymotrypsin, and plasminogen activator inhibitor 1 (3‑5). KLK2 is structurally related to KLK3, the prostate-specific antigen (PSA). Like PSA, KLK2 is considered to be a biomarker for prostate cancer (6).

References
  1. Chapelaine, P. et al. (1988) FEBS Lett. 236:205.
  2. Frenette, G. et al. (1997) Int. J. Cancer 71:897.
  3. Deperthes, D. et al. (1995) Biochim. Biophys. Acta 1245:311.
  4. Grauer, L.S. et al. (1998) J. Androl. 19:407.
  5. Mikolajczyk S.D. et al. (1999) Cancer Res. 59:3927.
  6. Sardana, G. et al. (2008) Clin. Chem. 54:1951.
Entrez Gene IDs
3817 (Human); 24841 (Rat); 102139474 (Cynomolgus Monkey)
Alternate Names
EC 3.4.21; EC 3.4.21.35; glandular kallikrein 2; Glandular kallikrein-1; hGK-1; hK2; Kallikrein 2; kallikrein 2, prostatic; kallikrein-2; kallikrein-related peptidase 2; Klk1c2; KLK2; KLK2A2; MGC12201; Tissue kallikrein-2; Ton

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Citation for Human Kallikrein 2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Direct B cell stimulation by dendritic cells in a mouse model of lupus.
    Authors: Wan S, Zhou Z, Duan B, Morel L
    Arthritis Rheum., 2008-06-01;8(0):1741-50.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization

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